通过DPR介导的H2O2耐药性有助于链球菌在囊性纤维化气道模型系统中的存活
Rendi R Rogers1, Christopher A Kesthely1, Fabrice Jean-Pierre1
1Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Journal of bacteriology
|June 28, 2024
概括
血球菌需要对反应性氧物种进行保护,以便在囊性纤维化肺部生物膜中生存. 与DNA结合的费里丁类蛋白Dpr和调节器PerR是这种保护的关键,特别是对Pseudomonas aeruginosa.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 生物膜研究 生物膜研究
背景情况:
- 囊性纤维化 (CF) 肺部感染涉及多微生物生物膜,影响患者的治疗结果.
- 链球菌 spp. 链球菌 spp. 在CF气道中很常见,但它们在疾病进展中的作用尚不清楚.
- 一个以前建立的CF多微生物培养模型允许研究物种间相互作用.
研究的目的:
- 为了确定能够使血流性球菌在CF多微生物群体中生存的遗传因素.
- 关于活性氧物种 (ROS) 的Streptococcus sanguinis和Pseudomonas aeruginosa之间的相互作用的研究.
主要方法:
- 使用了四种病原体CF多微生物培养系统.
- 进行了基因研究,以确定S. sanguinis.中的关键生存基因.
- 评估了Dpr和PerR在S. sanguinis对ROS和P. aeruginosa的保护中的作用.
主要成果:
- 鉴定了与活性氧物种 (ROS) 相关的基因,这些基因在S. sanguinis单一培养与多微生物生长中表达不同.
- 发现DPR是一种与DNA结合的费里类蛋白质,对于S. sanguinis在多微生物群体中的生存至关重要.
- Dpr及其调节器PerR保护S. sanguinis免受来自Pseudomonas aeruginosa和过氧化暴露的毒性.
结论:
- 在CF多微生物生物膜中,对活性氧物种的保护对于Streptococcus sanguinis的生存至关重要.
- Dpr-PerR系统对于S. sanguinis在CF肺环境中承受氧化应激至关重要.
- 这项研究提高了对Streptococcus spp.的理解. 在复杂的CF肺微生物组中的相互作用.
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