用Trichoderma viride进行预处理:为了更好地了解其对无氧消化过程的影响
Rudolf Markt1, Eva Maria Prem1, Nina Lackner1
1Department of Microbiology, Universität Innsbruck, Innsbruck, Austria.
Environmental microbiology reports
|June 28, 2024
概括
用Trichoderma viride对纤维素的真菌预处理略有减少了基质质量,但没有显著影响甲产量. 然而,它改变了无氧消化过程中的有机酸积累和微生物社区动态.
科学领域:
- 生物能源和生物燃料的使用
- 微生物生态学 微生物生态学
- 生物技术是生物技术.
背景情况:
- 优化生物预处理是第二代生物燃料生产的关键.
- 纤维素的真菌预处理提供了一个有希望的途径,但它的机制需要阐明.
研究的目的:
- 研究用Trichoderma viride进行有氧预处理对纤维素可降解性的影响.
- 评估这种预处理对无氧消化参数和微生物群落的影响.
主要方法:
- 使用Trichoderma viride进行晶体纤维素的有氧预处理.
- 批量发酵用于无氧消化.
- 戈珀茨建模用于动力分析.
- 16S rRNA amplicon测序和ANCOM-BC用于微生物社区分析.
主要成果:
- 菌预处理导致基质质量略有减少,但没有显著影响整体甲产量.
- 有机酸积累和降解动态,包括甲生产动态,受到预处理的影响.
- 微生物群落的组成,包括发酵性,合成性和甲原性类型,因治疗前的持续时间而显著改变.
结论:
- 用T. viride进行有氧预处理会影响无氧纤维素水解和甲生成活性.
- 这项研究为真菌预处理对无氧消化参数的直接影响提供了新的见解.
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