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无标签监测培系统动态:通过红外光谱成像检测益生菌和癌细胞相互作用

Yoon Jeong^1,2,3, Pei-Hsuan Hsieh^1,4, Yamuna Phal^5,6

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Analytical chemistry

|June 28, 2024

概括

这项研究引入了一种新的水凝平台,使用红外光谱学观察微生物与宿主相互作用. 它揭示了益生菌细菌如Lactococcus lactis如何影响人类癌细胞,为细胞动态提供了洞察力.

科学领域:

生物物理学的生物物理.
微生物学微生物学
细胞生物学细胞生物学

背景情况:

评估微生物与宿主细胞相互作用需要用于培养研究的先进平台.
益生菌细菌,如Lactococcus lactis,可以影响哺乳动物细胞的行为,包括通过像尼这样的分泌分子诱导亡.

研究的目的:

开发和验证一个无标签的红外光谱成像平台,用于动态微生物与宿主细胞相互作用分析.
为了研究A498人类癌细胞与益生菌菌共同培养的生物化学和细胞反应.

主要方法:

使用基于水凝的隔间系统对细菌 (乳糖杆菌,大肠杆菌) 和A498癌细胞进行受控的共同培养.
采用中红外 (IR) 光谱成像用于无标签的生物化学成分 (核酸,蛋白质,脂质) 的表征.
应用多变量回归建模,将光谱特征与细胞动态以及尼对癌细胞的影响相关联.

主要成果:

通过红外光谱学成功监测了A498细胞在与细菌共同培养期间的生物化学变异.
确定了预测细胞反应的关键光谱特征 (不和脂肪酸,蛋白质β转换,核酸).
量化了L. lactis分泌的尼对A498癌细胞的影响.

结论:

红外光谱成像是一种强大的无标签工具,用于研究复杂的微生物与宿主相互作用.
开发的平台可以对跨物种生理过程进行机械研究.
这种方法为益生菌对癌细胞的影响提供了宝贵的见解.

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