使用LC-MS/MS在人体血中的thiorphan的量化,并将其应用于生物等价性研究
Rana Said1, Basel Arafat2, Tawfiq Arafat3
1Pharmacological and Diagnostic Research Centre (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan.
概括
一种新方法准确地测量了人体血中racesacadotril的活性代谢物thiorphan. 这项验证的试验支持racecadotril用于治疗严重腹的药理动力学研究.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 生物分析是一种生物分析.
背景情况:
- 拉赛卡多特里尔是一种抗分泌药物,用于治疗严重腹的口服补水疗法.
- 尔方是racesacadotril的活性代谢物,负责其治疗作用.
- 精确量化血中的硫对于药理动力学和生物等价性研究至关重要.
研究的目的:
- 开发和验证一种灵敏和快速的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于量化人体血中的硫.
- 在种族cadotril. 的生物等价性研究中利用这种方法.
主要方法:
- 用LC-MS/MS分析人类血样本.
- 用蛋白沉来提取样本.
- 在InertSil CN-3列上进行了染色分离.
- 甲-d7作为内部标准.
主要成果:
- 该方法表现出极好的线性 (1-200 ng/ml,R2 ≥0.9991) 和精度 (日内和日间<10.0%).
- 分析时间很快,每个样本不到1分钟.
- 该方法被证明是稳定的,适合生物等价性研究.
结论:
- 成功开发了一种完全验证的,高效的,快速的LC-MS/MS方法,用于在人体血中定量硫.
- 该方法适用于支持racecadotril配方的药理动力学和生物等价性评估.
关键词:
生物等价性 生物等价性在HPLC中使用HPLC.在 LC MS/MS 中,蛋白质沉是蛋白质的沉.赛车cadotril的赛车cadotril是什么意思尔方的代谢物是其代谢物.验证 验证 验证 验证更多相关视频
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