在使用三种不同的液态染色学系统时,二聚体构成对酸聚核酸的染色学保留的影响
Stilianos G Roussis1, W Brad Wan1, Claus Rentel1
1Ionis Pharmaceuticals, 2855 Gazelle Ct., Carlsbad, California 92010, United States.
Journal of chromatography. A
|June 28, 2024
概括
本研究使用三种染色学方法对酸 (PS) 寡核酸进行了表征. 富含sp的双立体同位素具有较长的保留时间,从而可以从染色学数据中确定双立体同位素的组成.
科学领域:
- 分析化学 分析化学
- 橄核酸的化学成分
背景情况:
- 酸 (PS) 寡核酸在治疗中至关重要.
- 了解它们的异体异体性质对于药物开发至关重要.
- 染色学方法为复杂的分析技术提供了可访问的替代方案.
研究的目的:
- 为了研究二聚体异构体特性对PS寡核酸的染色学保留的影响.
- 为了比较三个不同的染色学方法的有效性,以表征立体丰富的PS化合物.
- 为了建立染色学概况和PS立体异构体组成之间的关系.
主要方法:
- 在系列逆相强离子交换 (RP-SAX) 染色学.
- 离子对逆相 (IP-RP) 染色学.
- 金属离子复合色谱 (MICC).金属离子复合色谱.
主要成果:
- RP-SAX和IP-RP产生了类似的二聚体异构体分布特征,显示了Sp-丰富的二聚体异构体以后被解离.
- 在RP-SAX和IP-RP的保留时间和RP/Sp PS链接的数量之间观察到线性关系.
- MICC表现出更广泛的分布,但与IP-RP相似的等级集群分析 (HCA) 分类,表明正交.
结论:
- 染色学方法,特别是RP-SAX和IP-RP,有效地表征了PS寡核酸二态异构体.
- 富含sp的双立体同位素表现出增加的相互作用,可能是由于更开放的配置.
- 染色学数据有可能通过进一步培训来确定PS寡核酸二聚体异构体的组成.
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