开发"智能颗粒"用于治疗牙腐烂
Yanping He1, Richard Bright2, Krasimir Vasilev2
1Adelaide Dental School, University of Adelaide, Adelaide, SA 5000, Australia.
概括
这项研究介绍了智能纳米粒子 (IPs) 用于牙损伤治疗. 这些适应pH的颗粒在酸性环境中有效向和消除空性细菌,为传统治疗提供了有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 牙科研究 牙科研究
背景情况:
- 牙损是一种普遍的全球性疾病,由生物膜中的酸性细菌引起.
- 目前的治疗方法面临着快速清除和选择性细菌向的挑战,以及日益增长的抗生素耐药性问题.
- 需要新的治疗策略,以有效地管理牙损伤,并保护口腔微生物群.
研究的目的:
- 开发和评估适应pH值的纳米颗粒,以有针对性地输送银纳米颗粒来对抗牙损伤.
- 创建智能颗粒 (IPs),它们与牙斑生物膜结合,并在酸性条件下释放治疗剂.
- 评估开发的纳米颗粒的抗菌膜有效性和细胞毒性.
主要方法:
- 在酸Fe(III) 复合修饰的PLGA粒子 (Fe(III) -TA/PLGA@BPEI-AgNPs) 中封装的分支PEI封顶银纳米粒子 (BPEI-AgNPs) 的合成.
- 对纳米颗粒与*S. mutans*生物膜结合的评估.
- 在不同的pH水平 (pH4.0与pH7.4) 上评估Ag+离子释放动力学.
- 在酸性条件下对*S. mutans*生物膜的抗菌膜活性进行体外测试.
- 使用Caco-2细胞和人类牙纤维细胞的细胞毒性测定.
主要成果:
- 构建的Fe(III) -TA/PLGA@BPEI-AgNPs (IPs) 表明它们对*S. mutans*生物膜具有显著的结合.
- 从IP中释放Ag+离子的速度较快,在pH值为4.0时与pH值为7.4.4相比,观察到pH值为4.0.
- 在酸性条件下,IPs显著降低了*S. mutans*生物膜体积和生存能力.
- 试验组对Caco-2细胞和人类牙纤维细胞没有显著的细胞毒性.
结论:
- 开发的智能颗粒 (IP) 显示出有针对性的牙损伤治疗的巨大潜力.
- IPs的pH响应性允许在空性口腔环境中局部释放药物.
- 专用药物提供了一种安全有效的策略,通过向致病生物膜,而不会伤害有益的口腔细菌或宿主细胞来对抗牙.
关键词:
生物膜是一种生物膜.牙腐烂是指牙的腐烂.细胞外多糖体 (EPS) 是一种多糖体.银纳米颗粒 银纳米粒子突变型链球菌 (Streptococcus mutans) 是一种突变型链球菌.响应pH的反应性更多相关视频
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