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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jun 22, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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开发了一种多模块组装原始编辑 (mPE) 系统,提高了双中精确的多基插入效率.

Pengjun Lu1, Erwei Zuo1, Jianbin Yan1

  • 1Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.

Journal of advanced research
|June 28, 2024
PubMed
概括
此摘要是机器生成的。

通过将系统分成一个多模块组装的主要编辑系统 (mPE) 来提高双中Prime Editing (PE) 的效率. 这种新的方法提高了精确的编辑和多基插入,克服了原来的PE架构的局限性.

关键词:
阿拉比多普西斯塔利亚纳.克里斯普尔是什么意思?克里斯普尔是什么意思?迪科特 (Dicot) 是一个名字.基因组编辑 基因组编辑尼科蒂亚纳 (Nicotiana benthamiana) 是一种植物.主要编辑主要编辑

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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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相关实验视频

Last Updated: Jun 22, 2025

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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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科学领域:

  • 植物合成生物学 植物合成生物学
  • 基因组编辑技术的技术
  • 分子生物学分子生物学

背景情况:

  • 总编辑 (PE) 是一种精确的基因组编辑工具,在植物育种和合成生物学中具有重大潜力.
  • 目前,双植物 (dicots) 的低PE效率限制了其应用.
  • 现有的PE系统在双中难以检测和不一致的编辑,阻碍了稳定的转换.

研究的目的:

  • 调查和解决 Prime 编辑在 dicots 中效率低的问题.
  • 开发和优化分割PE系统,以提高双植物的性能.
  • 为了确定最佳的分裂PE配置,以增强双中的基因编辑.

主要方法:

  • 在Arabidopsis thaliana和Nicotiana benthamiana中进行了大规模的Agrobacterium介导的转化实验.
  • 使用深度安普利康测序 (0.2-0.5百万清洁读数) 来分析编辑效率.
  • 进行了分裂PE组件的系统评估,包括融合逆转录酶和扩展的pegRNA.

主要成果:

  • 原始的融化PE架构显示在dcots中编辑效率降低.
  • 开发了一种新的多模块组装主要编辑系统 (mPE),显示了显著提高的编辑效率.
  • mPE实现了编辑效率的提高,从1.3倍到1288.5倍,使以前无法访问的位置进行编辑,并在多基插入方面表现出色 (平均提高了197.9倍).

结论:

  • 原始的PE架构对双的Cas9裂变活动产生了负面影响.
  • 分开PE系统,特别是开发的mPE系统,大大提高了双植物的编辑效率.
  • mPE系统在引入小插件方面特别有前途,突出了不同PE变体的专业功能.