类似受体的激酶ERECTA在葡萄中负面调节了氨酸的积累
Bo Wang1, Qianqian Pang2, Yunzhi Zhou1
1College of Agriculture, Guangxi University, No. 100, Daxue Road, Nanning, Guangxi 530004, China.
概括
作为一种类似受体的激酶,VvER 抑制了安东氨酸的合成,并降低了葡萄的抗寒性. 这种激酶负面调节了参与抗生素生产的关键基因,使葡萄更容易受到寒冷压力的影响.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 生物化学 生物化学
背景情况:
- 类似受体的激酶 (ERECTA家族) 在植物生长,发育和应激反应中起着至关重要的作用.
- 安西安素是重要的植物颜料,参与各种生理过程和耐压力.
- 了解氨酸合成的调节因素对于作物改进至关重要,尤其是在应对环境压力的情况下.
研究的目的:
- 为了研究VvERECTA (VvER) 在调节在冷却压力下葡萄中的安托氨酸生物合成中的作用.
- 阐明VvER影响植物中安东氨酸合成和抗寒性的分子机制.
- 评估VvER在异构系统中的功能,如Arabidopsis和草.
主要方法:
- 对抗生素生物合成途径基因和转录因子的基因表达分析 (qRT-PCR).
- 测量抗生素含量,素含量和抗氧化酶活性 (SOD,POD).
- 酵母两杂交测试以确定蛋白质与蛋白质之间的相互作用.
- 在草果实中进行过渡表达测试和在Arabidopsis中进行稳定转化.
主要成果:
- 葡萄中VvER的过度表达降低了关键的安东合成基因 (例如VvCHS,VvDFR) 和转录因子 (例如VvMYBA1,VvMYB5b) 的表达,抑制了安东合成和抗寒能力.
- 在葡萄中,VvER降低了proline含量和抗氧化酶活性 (SOD,POD).
- 在转基因阿拉比多普西斯中,VvER促进了花和树枝的延长,但在强光下抑制了花素的积累.
- 在草中,VvER通过抑制关键基因表达,在低温 (10°C) 下抑制了氨酸合成.
- 酵母两杂交试验证实了VvER与转录因子VvMYBA1,VvMYB5b和VvWDR1.1之间的相互作用.
- 通过降低MBW复合物的活性,VvER抑制了VvUFGT促进体活性,并降低了氨酸生物合成基因表达.
结论:
- 在葡萄中,VvER充当了素生物合成的负调节剂.
- VvER通过调节氨酸合成和相关的代谢途径,显著影响植物的抗寒能力.
- 这些发现表明VvER在使葡萄植物易受寒冷压力的作用,这与中国北部等地区的种植有关.
更多相关视频
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
10.5K
04:11Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
13.2K
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
MAPK Signaling Cascades
5.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.4K
Photoreceptors and Plant Responses to Light
20.3K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.3K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Fruit Development, Structure, and Function
22.2K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
22.2K
