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机械学研究的同位素标签

Houchao Xu1, Jeroen S Dickschat1

  • 1Kekulé-Institute for Organic Chemistry and Biochemistry, Rheinische Friedrich-Wilhelms-University of Bonn, Bonn, Germany.

Methods in enzymology
|June 28, 2024
PubMed
概括

同位素标记实验与重组合成酶相结合,为复杂的烯生物合成提供了强大的洞察力. 这种复兴的方法阐明了天然产品化学中的酶机制.

关键词:
绝对配置的确定绝对配置的确定.生物合成生物合成碳酸的化学成分酶的机制 酶的机制同位素标签的标签核磁共振光谱法 (NMR) 是一种光谱法.有氧烯铁酸盐的酸盐.再质子化是一种重质子化.立体选择性合成烯合成酶是一种烯合成酶.

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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