通过使用基质类似物的化学酶合成产生非自然类
Prabhakar L Srivastava1, Luke A Johnson1, David J Miller1
1School of Chemistry, Cardiff University, Cardiff, United Kingdom.
Methods in enzymology
|June 28, 2024
概括
我们开发了一种化学酶方法来合成新型类化合物,包括一种新的虫吸引剂. 这种方法扩大了类的化学多样性,为潜在的新应用提供了机会.
科学领域:
- 生物催化和有机合成
- 自然产品化学 自然产品化学
- 代谢工程是代谢工程.
背景情况:
- 类是一种具有广泛生物活性的多样化自然产品.
- 化学酶合成提供了一条强大的途径,可以访问新的类结构.
- 烯合成酶表现出乱活动,使得非自然化合物的产生.
研究的目的:
- 开发一种化学酶方法来合成非自然类.
- 为了产生一种新的虫吸引剂, (S-14,15-dimethylgermacrene D.
- 探索工程生物催化路径的潜力,用于化物生产.
主要方法:
- 使用了五种酶级联来合成化物.
- 使用Plasmodium falciparum胆酶和Methanocaldococcus jannaschii异丁酸盐酶进行二酸化.
- 使用Geobacillus stearothermophilus (2E,6E) -farnesyl二酸盐合成酶和Solidago canadensis (S) -germacrene D合成酶用于链延长和循环.
主要成果:
- 成功合成了新的虫吸引剂 (S-14,15-dimethylgermacrene D. D.)
- 证明了使用工程生物催化路径将前醇转化为类的有效转化.
- 生产了 (S) - 细菌D和新型二甲基化衍生物.
结论:
- 开发的化学酶法为新型非自然类提供了可访问的途径.
- 这种方法可以适用于合成各种类同类物和衍生物.
- 合成的化合物有可能在各个领域有新的应用.
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