细胞周期长度控制了非哺乳动物脊椎动物在早期发育期间的异染色蛋白重编程
Hiroto S Fukushima1,2, Takafumi Ikeda3,4,5, Shinra Ikeda3
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, 113-0033, Japan. hiroto.fukushima@riken.jp.
EMBO reports
|June 28, 2024
概括
基因组H3氨酸9三甲基化 (H3K9me3) 被删除并在受精后重新建立. 细胞周期长度控制了这种关键的表观遗传重编程在不同物种的早期发育.
科学领域:
- 表观遗传学和发育生物学
- 染色体生物学 染色体生物学
- 基因调节的分子机制
背景情况:
- 包括H3K9me3在内的异染色素标记在胚胎早期发育过程中受到动态调节.
- 正确的H3K9me3重编程对于成功的发育至关重要,但机制在物种之间可能有所不同.
- 之前的研究强调了无脊椎动物和非哺乳动物脊椎动物中保存的H3K9me3动态.
研究的目的:
- 为了阐明管理H3K9me3动态的分子机制在Medaka (Oryzias latipes) 作为一个模型的非哺乳动物脊椎动物.
- 调查细胞循环进展在H3K9me3清除和重新积累中的作用.
- 为了确定细胞周期长度依赖的H3K9me3重编程是否保留在其他物种中.
主要方法:
- 在Medaka早期发育期间分析H3K9me3动态.
- 研究细胞周期长度与H3K9me3水平之间的相关性.
- 在斑马鱼和Xenopus laevis中对H3K9me3重编程的评估与细胞周期长度相关.
主要成果:
- 梅达卡分裂阶段的快速细胞循环导致依赖于DNA复制的被动H3K9me3清除.
- 细胞循环的减缓,使细胞向中叶芽细胞过渡,允许Setdb1的积累和H3K9me3的重新建立.
- 发现细胞周期长度控制了斑马鱼和Xenopus laevis中的H3K9me3重编程.
结论:
- 细胞周期长度是H3K9me3在脊椎动物早期发育中的消灭和重新积累的关键决定因素.
- 在快速分裂的无脊椎动物和非哺乳动物的脊椎动物中存在着对H3K9me3重编程的保存,细胞周期长度依赖的机制.
- 这种机制在各种物种中得到保护,包括Drosophila,C. elegans,Xenopus,teleost鱼和medaka.
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