硫酸盐成像识别结直肠癌中瘤细胞 腹转移
G M Sarcinelli1, L Varinelli2, S Ghislanzoni1
1Department of Diagnostic Innovation, Fondazione IRCCS Istituto Nazionale Dei Tumori, Via G. Amadeo 42, 20133, Milan, Italy.
Cancer & metabolism
|June 29, 2024
概括
矩阵辅助激光溶解/电离 - 飞行时间 (MALDI-TOF) 影像检测在结直肠癌 (CRC) 周周转移 (PM) 中发现了独特的脂质谱. 葡萄糖脂硫酸盐 (STs) 标记瘤细胞,而特定的酸丁醇 (PI) 被改变,为PM进展提供了潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 结肠直肠癌 (CRC) 中的腹转移 (PM) 是常见的,尽管进行了积极的治疗,但预后不佳.
- 脂质在瘤生长和攻击性中起着至关重要的作用.
- 矩阵辅助激光消耗/电离 - 飞行时间 (MALDI-TOF) 质谱 (MS) 是识别生物标记物的强大工具.
研究的目的:
- 使用MALDI-TOF MS和MALDI-TOF成像MS (MALDI-IMS) 调查患者衍生瘤器官 (PDO) 和临床PM样本的脂质资料.
- 为了确定PM组织内脂质的组织分布.
- 为了确定PM进展的潜在的基于脂质的治疗点.
主要方法:
- 使用 MALDI-TOF MS 和 MALDI-IMS 在 PDO 和临床 PM 样本上.
- 分析了脂质样本及其在瘤和肌区内的空间分布.
- 量化特定的脂类物种,包括糖脂硫酸盐 (ST) 和酸丁醇 (PI).
主要成果:
- 在PM组织内的瘤细胞中鉴定出一种独特的脂质特征,主要是硫酸盐 (ST),在树突区域缺失.
- 含有阿拉基酸 (AA) 的酸氨基 (PI) 在树皮成分中表达高.
- 与树皮细胞相比,瘤细胞显示PI物种的丰度更高,具有更短,更和的乙烯链 (C34和C36).
结论:
- 独特的脂质特征,特别是改变的酸丁醇 (PI) 和硫酸盐 (ST) 物种,表明细胞颠覆促进瘤生长,攻击性和转移.
- 涉及葡萄糖脂 (GSL) /STs的PM代谢编程呈现了潜在的新疗法点,以抑制PM进展.
更多相关视频
10:58Quantitation of Intra-peritoneal Ovarian Cancer Metastasis
Published on: July 18, 2016
10.9K
08:47Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
Published on: August 21, 2012
17.3K
相关概念视频
01:26Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy
72
This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...
72
01:25Imaging Studies II: Positron Emission Tomography and Scintigraphy
105
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
Fundamental Principles of PET
105