循环应变对3D人体肌肉培养模型中的弹性合成的影响
Carolyn A Nietupski1, Andreja Moset Zupan1, Stacey C Schutte1
1Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio, USA.
Tissue engineering. Part C, Methods
|June 29, 2024
概括
机械拉伸,而不是荷尔蒙,在工程性子宫组织中显著增加了弹性纤维的产生. 特定的压力频率和振幅是刺激热素和纤维素-1合成的关键.
科学领域:
- 生物医学工程 生物医学工程
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 弹性纤维合成对于原生组织功能至关重要,但在工程组织中具有挑战性.
- 了解弹性生成调节对于推进组织工程方法至关重要.
- 怀孕涉及弹性纤维的增加,这表明机械拉伸和激素的作用.
研究的目的:
- 调查机械拉伸参数和怀孕水平激素对人类子宫肌肉平滑肌肉细胞弹性纤维生产的作用.
- 在3D培养模型中确定应变幅度和频率如何影响弹性生成.
- 评估机械应变和激素对弹性质和相关基因表达的联合影响.
主要方法:
- 人类子宫肌肉平滑肌细胞在3D模型 (肌肉组织结构,MTC) 中培养.
- 在6天内,MTC接受了各种周期性应变幅度 (5-15%) 和频率 (0.1-1赫兹),有或没有怀孕水平激素.
- 试验包括细胞增殖,弹性蛋白质含量,以及托波拉斯 (ELN) 和纤维素-1 (FBN1) 的基因表达.
主要成果:
- 一个0.5赫兹频率和10%的菌株振幅显著增加了弹性蛋白和ELN/FBN1mRNA表达.
- 增加应变幅度 (15%) 或频率 (1 Hz) 也增强了弹性纤维组件合成.
- 怀孕模仿激素治疗对弹性纤维生产没有显著影响.
结论:
- 仅仅机械应变就足以在这个3D子宫光滑肌细胞模型中刺激弹性纤维的产生.
- 应变幅度和频率是调节弹性生成的关键参数.
- 在怀孕引起的变化期间,激素可能不是调节弹性质合成的主要因素.
相关概念视频
Elastin is Responsible for Tissue Elasticity
Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
Relaxation of Skeletal Muscles
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
Deformation of Member under Multiple Loadings
When a rod is made of different materials or has various cross-sections, it must be divided into parts that meet the necessary conditions for determining the deformation. These parts are each characterized by their internal force, cross-sectional area, length, and modulus of elasticity. These parameters are then used to compute the deformation of the entire rod.
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...


