内子的分类学和小内子的进化
Anouk M Olthof1, Charles F Schwoerer1, Kaitlin N Girardini1
1Physiology and Neurobiology Department, University of Connecticut, Storrs, CT, USA.
Nucleic acids research
|June 29, 2024
概括
研究人员将内子重新分类,超出了传统的主要和次要类别,引入了新的分类,如次要类型和主要类型的内子. 这一修订后的系统有助于理解内部进化和拼接机制.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 内部的分类历史上一直是二进制的 (主要和次要的内部).
- 存在广泛的内部共识序列,挑战二进制模型.
- 了解内部进化和拼接在分子生物学中至关重要.
研究的目的:
- 根据更广泛的共识序列来重新分类内子.
- 研究新发现的内子类型的进化作用,例如小类内子.
- 阐明这些多样化的内子类的拼接机制.
主要方法:
- 对263个物种和6个真核生物超群的内部正统学分析.
- 内部共识序列的比较序列分析.
- 综合分析使用CoLa-seq,CLIP-seq (主要和次要结合体组件) 和RNAseq (具有次要结合体抑制).
主要成果:
- 建议对内子进行新的分类:小,小类,混合,大类,大类和非正规.
- 类似次要的内子被认为是内子进化中的过渡形式.
- 类似小的内子表现出小内子和大内子的特征,在拼接中作为中间类运作.
- 确定了使小类内子中主要和次要结合酶体能够识别的序列元素.
结论:
- 拟议的修订后的内部分类为研究内部进化和拼接提供了一个新的框架.
- 类似次要的内子代表了内子进化转化中的关键中间阶段.
- 这项研究揭示了基于序列的机制,这些机制是不同的结合酶体对小类型内子的双重识别的基础.
相关概念视频
Organization of Genes
68.5K
Overview
68.5K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K
Genome Size and the Evolution of New Genes
7.9K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
7.9K
Eukaryotic Evolution
33.4K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
33.4K
RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K


