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Updated: Jun 22, 2025

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通过EF-P和ABCF ATPases YfmR和YkpA/YbiT解决核糖体停滞

Hiraku Takada^1,2,3, Keigo Fujiwara¹, Gemma C Atkinson^3,4

¹Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University, Kamigamo, Motoyama, Kita-ku, Kyoto 603-8555, Japan.

Nucleic acids research

|June 29, 2024

概括

细菌亚细菌ABCF ATPases帮助核糖体合成困难的蛋白质序列. YfmR有助于普罗林丰富的动机翻译,而YkpA/YbiT有助于充电的氨基酸延伸.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

蛋白质合成效率受到氨基酸序列的影响.
富含和充电的氨基酸延伸在翻译过程中带来了挑战.
假设ATP结合盒F (ABCF) 的ATP酶有助于翻译困难的图案.

研究的目的:

描述四种家庭ABCF ATPases (YdiF,YfmM,YfmR/Uup,YkpA/YbiT) 在Bacillus subtilis蛋白质合成中的作用.
研究ABCF ATPases与EF-P等转化因子之间的相互作用.

主要方法:

基因分析基因分析
基于记者的测试分析.
在生物体内进行的研究.

主要成果:

发现YfmR与EF-P合作翻译富含proline的动机,同时损失导致严重的生长缺陷.
经过修改的EF-P变体的过度表达抑制了由YfmR和EF-P损失引起的生长缺陷.
YfmR过度表达缓解了Asp-Pro动机上的核糖体停滞.
YkpA/YbiT被证明可以促进充电动图的翻译,但不是富含proline的动图.

结论:

家政 ABCF ATPases在克服B. subtilis的特定翻译挑战方面发挥着独特的作用.
YfmR和EF-P对于高效的普罗林丰富动机转换至关重要.
YkpA/YbiT促进了带有充电氨基酸序列的蛋白质的合成.

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