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[来自Bacillus pumilus 3-19的细胞外膜囊泡的蛋白质组]

W Kurdy¹, P V Zelenikhin¹, G Yu Yakovleva¹

¹Kazan (Volga-Region) Federal University, Kazan, 420008 Russia.

Molekuliarnaia biologiia

|June 29, 2024

概括

来自Bacillus pumilus 3-19的细胞外膜囊泡被分析,没有发现任何遗传物质. 蛋白质组成因酸盐含量而异,酸盐缺乏导致囊泡中的双酶分泌.

科学领域:

微生物学微生物学
细菌生理学细菌生理学
细胞外囊泡细胞外囊泡

背景情况:

细胞外膜囊泡 (EMVs) 对于细菌通信和宿主相互作用至关重要.
电磁体载有调节分子,显示出作为生物标志物和治疗药物的潜力.
只有少数细菌物种的囊泡组成是已知的.

研究的目的:

为了隔离和特征EMVs从巴西杆菌3-19,一种抗 estreptomycin 的菌株.
分析这些EMV的基因组和蛋白质组合.
为了研究酸盐度对EMV组成的影响.

主要方法:

来自B. pumilus 3-19的EMV的隔离和可视化.
孤立EMV的基因组和蛋白质组分析.
来自缺和高酸盐介质的EMV的比较分析.

主要成果:

在B. pumilus 3-19 EMV中没有检测到遗传物质.
囊泡蛋白质组合因酸盐水平而有显著的变化.
缺乏的中型EMV含有49种独特的蛋白质,而高的EMV含有101种;140种蛋白质是常见的.
确定的特定蛋白质包括鞭毛蛋白,RNase J,酸酶,酸酶和铁载体.
酸盐缺乏引起的双酶仅在来自酸盐缺乏介质的EMV中发现.

结论:

B. pumilus 3-19 EMV缺乏遗传物质,其蛋白质组是由环境酸盐调节的.
酸盐的可用性影响EMV的货物,影响它们的功能作用.
鉴定到的蛋白质表明,这些囊泡在细菌生理学和相互作用中具有不同的功能.

关键词:

菌菌 (Bacillus pumilus) 是一种菌.在RNase中使用RNase.粉样样蛋白质是一种类似粉样蛋白质的蛋白质.这里是BinaseBinaseBinase 细胞外膜囊泡中的细胞外膜囊泡.鞭毛细胞中的蛋白质.基因组基因组是基因组的组成部分.蛋白质组是蛋白质组的组成部分.

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