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¹³C NMR: ¹H–¹³C Decoupling01:04

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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分离蛋白质度和聚合物含量使用扩散和水NMR.

Mark I Grimes1, Matthew Cheeks2, Jennifer Smith2

  • 1Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge CB3 0AS, U.K.

Analytical chemistry
|June 29, 2024
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概括

水的NMR (wNMR) 现在可以分离蛋白质度和聚合水平. 通过测量水扩散 (D) 与放松率 (R2) 一起,该方法为生物过程监测提供了一个新的工具.

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科学领域:

  • 生物制药学分析分析
  • 过程分析技术 (PAT) 是一种分析技术.
  • 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学

背景情况:

  • 基于蛋白质的药物,如单克隆抗体,是全球领先的制药品.
  • 监测蛋白质度和聚合物水平对于生物过程控制至关重要.
  • 使用横向放松率 (R2) 的水NMR (wNMR) 可以测量蛋白质度和聚合物,但无法区分它们.

研究的目的:

  • 开发一种方法来"解"并同时确定生物制药中的蛋白质度和总量水平.
  • 克服现有的wNMR技术的局限性,即无法区分这两个关键参数.

主要方法:

  • 使用的水扩散系数 (D ((H2O)) 测量与水横向放松率 (R2 ((H2O)) 来自wNMR.
  • 在三个不同的蛋白质系统上演示了该方法.
  • 涉及到特定于感兴趣的蛋白质的D (H2O) 和R (H2O) 的校准数据采集.

主要成果:

  • 成功解并同时确定蛋白质度和聚合物水平.
  • 结合D ((H2O) 和R2 ((H2O) 的方法即使在单个参数重叠的情况下也有效.
  • 在各种蛋白质系统中验证了该方法.

结论:

  • 组合的wNMR方法 (D ((H2O) + R2 ((H2O)) 有效地分离了蛋白质度和总量水平.
  • 这种技术显示了作为在线过程分析技术的实施前景.
  • 能够更精确地监测和控制基于蛋白质的治疗方法的生物过程.