从Polygonum Cuspidatum中以离子液为基础的超声波酶辅助提取复星的工艺条件的优化
Hongyi Zhao1, Junping Wang1, Yutong Han1
1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, PR China.
Ultrasonics sonochemistry
|June 29, 2024
概括
这项研究优化了使用离子液体和超声波酶辅助提取 (UEAE) 来从Polygonum Cuspidatum中提取复星. 新的绿色溶剂系统实现了2.90mg/g的高产量,证明了简化和更安全的提取过程.
科学领域:
- 自然产品化学 自然产品化学
- 绿色化学 绿色化学
- 生物技术是生物技术.
背景情况:
- 复星是一种强大的抗氧化剂,可以在Polygonum Cuspidatum (P.Cuspidatum) 中找到.
- 有效和环保的提取方法对于从植物来源获得复星至关重要.
- 传统的提取方法通常涉及苛刻的化学品和低产量.
研究的目的:
- 开发一种生产性和绿色的技术,从P. Cuspidatum中提取复星.
- 使用统计和计算模型优化提取参数.
- 评估不同优化模型的预测性能.
主要方法:
- 离子液体被选出它们在提取中的有效性.
- 使用了超声波酶辅助提取 (UEAE).
- 普莱克特-伯曼设计 (PBD) 确定了关键参数.
- 响应表面方法 (RSM) 和人工神经网络-遗传算法 (ANN-GA) 用于优化.
主要成果:
- 确定了最佳条件:2.18%的酶,58°C,29毫升/克的液体固体比,pH值5.5,30分钟,250瓦的超声波功率和0.5mol/L的1--3-甲基化物.
- 实现了2.90±0.15毫克/克的复星产量.
- 与RSM模型相比,ANN-GA模型显示出更高的预测准确性.
结论:
- 使用离子液体开发的UEAE方法在提取白醇方面非常有效.
- 新的绿色溶剂系统提高了提取效率,安全性和可行性.
- ANN-GA方法为优化天然产品提取过程提供了一个强大的工具.
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