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猪胚胎的玻璃化破坏了微RNA转录组配置的调节

Cristina Cuello¹, Alejandro González-Plaza¹, Josep M Cambra²

¹Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research "Campus Mare Nostrum", Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain.

|June 29, 2024

概括

使用SOPS或Cryotop方法进行猪芽细胞体的玻璃化改变了microRNA (miRNA) 的表达,影响了基因和细胞过程,如增殖和亡. 这两种玻璃化技术之间存在差异.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

玻璃化是胚胎的一个关键冷保存技术.
了解不同玻璃化方法对分子的影响对于优化辅助生殖技术至关重要.
微RNAs (miRNAs) 在胚胎发育过程中在调节基因表达方面发挥着至关重要的作用.

研究的目的:

为了比较超细开拉吸管 (SOPS) 和Cryotop玻璃化方法对猪母细胞体miRNA转录组的影响.
为了研究改变miRNA表达和化后目标基因 (TG) 表达变化的关系.
为了确定受玻璃化诱导的miRNA失调影响的特定分子通路.

主要方法:

使用SOPS或Cryotop方法进行玻璃化,并在体外培养猪胚胎囊.
使用微阵列进行了微RNA表达概况分析.
进行了miRNA和mRNA转录组数据的综合分析,以确定差异表达的基因和途径.

主要成果:

与对照组相比,SOPS和Cryotop玻璃化都显著改变了miRNA表达特征.
低温玻璃化产生了比SOPS (94) 更多的差异表达miRNAs (174).
玻璃化影响了参与TGF-β信号传递 (SOPS) 和Notch信号传递 (Cryotop) 等途径的基因.

结论:

使用SOPS和Cryotop方法的玻璃化诱导了猪胚胎囊中的miRNA失调,具有方法特异性的差异.
改变的miRNAs主要与细胞增殖,细胞亡和应激反应有关.
需要进一步的研究来阐明TGF-β和Notch途径改变对胚胎发育的影响.

关键词:

胚胎囊中的胚胎细胞.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪目标基因是目标基因.转录组转录组就是一个转录组.玻璃化是一种玻璃化过程.这是一个小RNARNA.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...