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红色化合物:在药理上诱导铁灭菌的潜在工件

Matías Vera¹, María José Barahona², Estefanía Nova-Lamperti³

¹Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepción, Concepción, Chile; Laboratory of Neurobiology and Stem Cells, NeuroCellT, Department of Cellular Biology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.

Free radical biology & medicine

|June 29, 2024

概括

细胞培养基中的红色可以错误地增加对某些铁灭诱导物的敏感性,如IKE和RSL-3. 这种pH指标会改变基因表达和蛋白质定位,使铁灭症研究复杂化.

科学领域:

细胞生物学细胞生物学
生物化学生化学
药理学药理学是指药理学的学科.

背景情况:

红 (PR) 是细胞培养基中常见的pH指标.
它对ferroptosis诱导的潜在干扰尚未得到充分理解.

研究的目的:

调查红是否影响细胞中的铁灭诱导.
阐明任何观察到的干扰背后的机制.

主要方法:

高含量活细胞成像死亡分析.
药理学诱导铁灭症使用伊米达基埃拉斯 (IKE),RSL-3和FINO2.
基因表达分析和超分辨率显微镜.

主要成果:

醇红色预先使细胞受到IKE和RSL-3诱导的铁亡,但不是FINO2.
PR降低了抗铁生菌基因 (Slc7a11,Slc3a2,Gpx4) 的水平,并增加了铁生菌诱导体Acls4.4.
PR导致了系统x_c^-从血中错位.

结论:

培养介质中的红色可以在铁死研究中成为一种令人困惑的工件.
它不准确地影响细胞对IKE和RSL-3等特定铁亡诱导物的敏感性.
研究人员应该考虑省略PR以准确评估铁亡.

关键词:

ACSL4 ACSL4 ACSL4 ACSL4 ACSL4 ACSL4 ACS 铁化是铁化的一种.在GPX4中使用GPX4.这里是伊基伊基伊基伊基.醇红色醇红色醇红在RSL-3中使用RSL-3.系统x (c) (−) 系统

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