BomMDH1 调节烟草 BY-2 悬浮细胞中马拉酸盐介导的氧化应激
Haohui Chen1, Shifeng Cao2, Jiahui Chen1
1College of Food Science and Engineering, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo, 315800, China.
Journal of plant physiology
|June 30, 2024
概括
菜是什么意思 菜是什么意思
科学领域:
- 植物分子生物学 植物分子生物学
- 细胞应激反应细胞应激反应
背景情况:
- 编程细胞死亡 (PCD) 对植物发育至关重要.
- "马拉酸"调节细胞的氧化还原平衡.
研究的目的:
- 调查西兰花BomMDH1基因在马拉特诱导的亡中的作用.
- 评估BomMDH1在减轻氧化应激中的功能.
主要方法:
- 使用转基因明亮黄色-2 (BY-2) 悬浮细胞过度表达BomMDH1.1.
- 测量了反应性氧物种 (ROS),过氧化 (H2O2) 和甲 (MDA) 的含量.
- 分析了抗氧化酶基因表达和细胞活力.
主要成果:
- 在氧化应激下,BomMDH1过度表达增强了BY-2细胞活力.
- 在转基因细胞中观察到减少的ROS,H2O2和MDA水平.
- 抗氧化基因 (NtAPX,NtAOX1a,NtSOD,NtMDHAR) 的升级调节,并通过SHAM治疗改善抗亡活性.
结论:
- 在植物中,BomMDH1对马拉酸盐诱导的亡和氧化应激起着保护作用.
- Salicylhydroxamic 酸 (SHAM) 显示出增强植物细胞应激耐受性的潜力.
相关概念视频
Regulation of Transpiration by Stomata
26.3K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
26.3K
Adaptations that Reduce Water Loss
24.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
24.4K


