在体外胃肠道消化过程中被封装在固体脂质颗粒中的氨基酸的释放特征
Sharmitha Rajendrakumar1, Valérie Beaumal1, Alice Kermarrec1
1INRAE, BIA, F-44316 Nantes, France.
Food research international (Ottawa, Ont.)
|June 30, 2024
概括
固体脂质颗粒保护氨基酸用于肠道微生物的新陈代谢. 颗粒的大小和结构,而不是酶,主要控制消化过程中的释放,影响潜在的健康益处.
科学领域:
- 动物营养 动物营养
- 胃肠道生理学 胃肠道生理学
- 药物输送系统 药物输送系统
背景情况:
- 氨基酸通过肠道微生物群代谢调节免疫反应.
- 饮食中的氨基酸主要在小肠中被吸收,限制了结肠微生物的访问.
- 封装旨在将氨基酸输送到结肠,以增强微生物代谢和健康益处.
研究的目的:
- 在模拟的小猪消化过程中,研究从固体脂质颗粒中释放的氨基酸.
- 描述固体脂质颗粒 (SLPs) 的组成,热行为,结构和形态.
- 确定颗粒大小和物理性质对氨基酸释放的影响.
主要方法:
- 在体外耳胃肠道消化模型模拟小猪生理学.
- 将氨基酸 (BCAA混合物, lysine 或 tryptophan) 装入SLP中.
- SLP的特征:组成,热分析,结构分析,形态,粒子大小分布.
- 根据SLP的大小分成部分和氨基酸释放的评估.
主要成果:
- 在胃阶段,氨基酸的释放量很高,受颗粒大小和晶体状态的影响.
- 较大的颗粒大小和更光滑,有序的表面导致氨基酸释放速度更慢和更低.
- 脂质水解发生在肠道阶段,但物理性质 (晶体状态,形态) 仍然影响通过扩散释放.
结论:
- 粒子大小和表面形态等物理参数显著控制消化过程中SLP从SLP释放的氨基酸.
- 水/溶液扩散是从多孔SLPs释放氨基酸的关键机制,独立于酶活性.
- 优化SLP的物理特性对于有针对性地将氨基酸输送到结肠以进行微生物发酵至关重要.
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