两种毒性沙门氏菌的特征和在蛋,肉类和生菜中暂时应用的安全性
XiaoWen Sun1, Fan Xue1, Cong Cong2
1MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Food research international (Ottawa, Ont.)
|June 30, 2024
概括
两种新型细菌菌体,sp11241和8-19,有效控制了在蛋,肉和生菜中的Salmonella enterica血清型Enteritidis (SE) 污染. 这些菌体有望成为食品安全应用中的绿色生物控制剂.
科学领域:
- 食品安全与微生物学
- 菌体研究 研究 菌体研究
- 抗微生物战略 抗微生物策略
背景情况:
- 沙门氏菌带来了严重的食物传播疾病风险,导致因残留问题而需要替代抗生素的替代品.
- 菌体正在成为对抗细菌病原体的环保生物控制剂.
研究的目的:
- 为了识别和表征新的毒性细菌菌对沙门氏菌 (Salmonella enterica) 血清型 Enteritidis (SE).
- 评估两个分离的菌体 (sp11241和8-19) 在低温下降低各种食品矩阵中SE污染的有效性.
主要方法:
- 隔离和识别了两个新的沙门氏菌:vB_SalS_ABTNLsp11241 (sp11241) 和8-19.
- 对菌体感染性对 Salmonella enterica 血清型 Enteritidis (SE) 的评估.
- 在4°C的SE污染的全蛋,生肉和生菜上对菌素有效性的实验性评估,使用不同的感染多重性 (MOI).
主要成果:
- 菌体sp11241和8-19证明了对SE的有效传染性.
- 在100和10000的MOI下,在2-6小时内实现了对蛋的完全SE消除.
- 在使用菌体sp11241.12小时后,在生肉 (高达3.17 log10 CFU/mL) 和生菜 (高达3.00 log10 CFU/mL) 上观察到SE水平的显著降低.
结论:
- 已识别的菌体sp11241和8-19在控制食品中的沙门氏菌污染方面是有效的.
- 这些菌体代表了低温食品应用的有希望的,安全的和绿色的抗菌剂,解决了抗生素耐药性的问题.
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