使用葡萄油和羊角酸进行MLM型结构性脂质的酶合成和表征
1Department of Food Science and Technology, University of Georgia.
Journal of oleo science
|June 30, 2024
概括
结构性脂质 (SL) 被优化为C10:0纳入葡萄油使用两种脂酶. 酶RM IM产生了更高的C10:0结合,导致具有潜在健康益处的功能MLM型SL.
科学领域:
- 脂质化学和酶学 脂质化学和酶学
- 食品科学与技术 食品科学与技术
- 生物催化剂是一种生物催化剂.
背景情况:
- 结构性脂质 (SL) 通过修改脂肪酸概况,提供潜在的健康益处.
- 葡萄油 (GS油) 是一种常见的食用油来源,适合修改.
- 优化C10:0等特定脂肪酸的纳入对于SL功能至关重要.
研究的目的:
- 为了优化C10:0在葡萄油中的反应条件.
- 描述由此产生的结构性脂质产品,重点关注TAG物种和脂肪酸位置.
- 评估SL合成和净化对抗氧化活性的影响.
主要方法:
- 采用了塔古奇法来优化反应参数 (基质比,酶负荷,温度,时间).
- 使用固定化脂酶进行了酸解反应:Lipozyme® RM IM和Lipozyme® 435.
- 高性能液体染色学 (HPLC) 和气体染色学 (GC) 用于分析;扩大反应和短路蒸 (SPD) 用于净化和表征.
主要成果:
- 对两种酶都确定了最佳条件 (1:3 GS油:C10:0比率,5%的酶负荷,65°C,12小时).
- 与Lipozyme® 435 (50.78 mol%) 相比,Lipozyme® RM IM的C10:0总合并量 (60.08 mol%) 显著更高.
- 纯化SL (72.96重量%的产量) 显示SPD后抗氧化活性降低,原因是托科菲醇损失,但保留了高C10:0含量的MLM型TAG结构.
结论:
- 利波胺® RM IM是一种优质生物催化剂,用于从葡萄油中产生高C10:0含量的MLM型结构性脂质.
- 优化的12小时过程产生了一个功能性的SL,具有潜在的营养和健康优势.
- 短路蒸有效净化SL,但需要考虑抗氧化成分的保留.
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