相关实验视频

Updated: Jun 22, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

在子宫纤维瘤中m6A修饰剂和RNA修饰物的表征

Jitu W George^1,2, Rosa A Cancino¹, Jennifer L Griffin Miller¹

¹Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE 68198, USA.

|July 1, 2024

概括

子宫纤维瘤 (leiomyomas) 在RNA修饰剂和N6-甲基氨酸 (m6A) 水平上显示出有限的变化. 需要对不同患者群体进行进一步的研究,以了解纤维瘤发育和表观遗传贡献.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子瘤学分子瘤学

背景情况:

子宫肌瘤 (纤维瘤) 是常见的良性瘤,其起源不清楚.
包括RNA甲基化在内的表观遗传修饰越来越多地与瘤发生有关.

研究的目的:

研究表观遗传媒介和RNA修饰的作用,特别是N6-甲基氨酸 (m6A),在子宫纤维瘤中.
识别差异表达的m6A修饰剂及其与纤维瘤亚型和驱动突变的关联.

主要方法:

RNA测序 (RNA-seq) 和RT-qPCR用于分析表观遗传媒介表达.
西方涂抹测试用于评估m6A修饰剂的蛋白质水平.
超高性能液态染色体质谱学 (UHPLC-MS) 用于量化m6A和其他RNA修饰.
对公开可用的数据集进行生物信息分析.

主要成果:

大多数测试的RNA修饰剂和m6A修饰的有限差异表达在纤维瘤和肌肉膜之间被观察到.
关键m6A修饰物的蛋白质水平与RNA表达数据一致.
在特定的纤维瘤遗传亚型中确定了一些m6A修饰物的差异表达.
在以前与纤维瘤发育有关的基因中发现了m6A共识动机.

结论:

虽然子宫纤维瘤中RNA修饰剂和m6A丰度的整体变化是有限的,但特定的变化可能与纤维瘤亚型有关.
纤维瘤的异质性质表明需要更大,多样化的队列来充分阐明m6A修饰在纤维瘤病理中的作用.

关键词:

标志性转录组学标志性转录组学竞争竞争竞争竞争这是子宫纤维瘤.

更多相关视频

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...