链逆转后珍珠催化键形成,由尿路形成的凝结域形成
Yue Yu1, Wilfred A van der Donk1
1Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
ACS central science
|July 1, 2024
概括
研究人员发现,先前未知的功能的兰西生物脱水酶可以形成键. 这一发现扩大了我们对天然产品生物合成和这些酶的多样性作用的理解.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 自然产品生物合成 自然产品生物合成
背景情况:
- 非核糖体合成酶 (NRPSs) 和多基合成酶 (PKSs) 经常与被注释为lantibiotic dehydratases的酶一起发现.
- 这些假定的兰西生物脱水酶的特定功能以前没有被阐明.
研究的目的:
- 描述一个NRPS-PKS生物合成基因集群 (BGC),其中含有来自*Stackebrandtia nassauensis*的假定兰地生物脱水酶.
- 为了阐明生物合成途径,并确定这个BCG的最终产品.
主要方法:
- 来自*S. nassauensis*的NRPS-PKS BGC的异构表达.
- 通过选择生物合成酶的省略进行代谢物分析和途径阐明.
- 酶活性位点的生物信息学分析,包括尿素生成凝结 (Curea) 领域.
主要成果:
- 在BGC产生一个bisarginyl ureidopeptide与一个enone电友.
- 假定的兰西生物脱水酶催化了键的形成,延长了基架.
- 发现了一种新的尿素群形成机制,由NRPS SnaA的凝聚域催化,具有明显的EHHXXHDG活性位点特征.
结论:
- 兰氏生物脱水酶与NRPS一起作为独立的胺键形成机制起作用.
- 兰西生物脱水酶酶家族在天然产品生物合成中表现出各种催化活动.
- 这项研究扩展了已知的兰西生物脱水酶的功能,揭示了它们在核糖体和非核糖体自然产物合成中的作用.
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