Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sparse Distributed Archetypes Reveal Compressible Network Motifs Underlying Naturalistic Cognition.

bioRxiv : the preprint server for biology·2026
Same author

Induced pluripotent stem cells as platforms for engineering NK cell immunotherapies.

Frontiers in cell and developmental biology·2026
Same author

Anti-BCMA-CAR-IL15 natural killer cells prevent multiple myeloma growth in the bone marrow but allow subsequent emergence of extramedullary disease.

Cancer letters·2025
Same author

Introduction to the Immune System.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells.

Antibody therapeutics·2025
Same author

Identifying Modifiers of CAR T-Cell Therapeutic Efficacy and Safety: A Systematic Review and Individual Patient Data Meta-Analysis.

Transfusion medicine reviews·2025

相关实验视频

Updated: Jun 11, 2026

Author Spotlight: High-Throughput Screening of CAR T-Cell Constructs for Enhanced Cytotoxicity and Immunologic Memory
09:20

Author Spotlight: High-Throughput Screening of CAR T-Cell Constructs for Enhanced Cytotoxicity and Immunologic Memory

Published on: October 27, 2023

2.3K

一种基于流细胞计的方法,用于评估使用稳定的CARjurkat细胞的CAR细胞结合动力学.

Alex Shepherd1,2, Bigitha Bennychen1,2, Zafer Ahmed1

  • 1Human Health Therapeutics Research Centre, National Research Council, Ottawa, ON, Canada.

Bio-protocol
|July 1, 2024
PubMed
概括

这项研究引入了一种简单的流动细胞计量试验,以测量嵌合抗原受体 (CAR) T细胞结合动力学. 该试验量化了CAR-目标细胞相互作用,有助于理解癌症治疗中的CAR功能.

关键词:
在CART-T中,使用CART-T.细胞对细胞的相互作用.细胞的贪性 细胞的贪性细胞动力学 细胞动力学流动细胞计量流动细胞计量高吞吐率的高吞吐量.朱尔卡特·朱尔卡特 (Jurkat Jurkat) 是一个名为朱尔卡特的地区.查检查 查检查 查检查一个T细胞细胞.

更多相关视频

Author Spotlight: Advancements in Hypoxia-Sensitive CAR-T Therapy for Enhanced Cancer Immunotherapy
09:12

Author Spotlight: Advancements in Hypoxia-Sensitive CAR-T Therapy for Enhanced Cancer Immunotherapy

Published on: June 14, 2024

880
Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

812

相关实验视频

Last Updated: Jun 11, 2026

Author Spotlight: High-Throughput Screening of CAR T-Cell Constructs for Enhanced Cytotoxicity and Immunologic Memory
09:20

Author Spotlight: High-Throughput Screening of CAR T-Cell Constructs for Enhanced Cytotoxicity and Immunologic Memory

Published on: October 27, 2023

2.3K
Author Spotlight: Advancements in Hypoxia-Sensitive CAR-T Therapy for Enhanced Cancer Immunotherapy
09:12

Author Spotlight: Advancements in Hypoxia-Sensitive CAR-T Therapy for Enhanced Cancer Immunotherapy

Published on: June 14, 2024

880
Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

812

科学领域:

  • 免疫学 免疫学 免疫学
  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学

背景情况:

  • 化学抗原受体 (CARs) 为癌症治疗重编程免疫细胞,特别是在血液性恶性瘤中.
  • 了解控制CAR反应的机制和结构性质对于优化其有效性至关重要.
  • 目前存在关于影响CAR介导细胞相互作用和治疗结果的确切因素的知识差距.

研究的目的:

  • 开发和验证一种简单的测定方法,用于评估CAR表达细胞的细胞性.
  • 描述CAR表达T细胞和向抗原表达细胞之间的相互作用动力学.
  • 为更好地了解CAR功能和信号事件提供一个工具.

主要方法:

  • 使用标准流动细胞计来测量表达CAR的Jurkat细胞和目标细胞之间的相互作用动力学.
  • 采用CAR Jurkat细胞与标阳性和标阴性细胞的共同培养,在不同的效应因子-标比率下.
  • 观察到CAR-目标细胞双胞胎的形成,作为积极结合的细胞的读数,不需要染色.

主要成果:

  • 开发了一种简化的试验,以量化CAR介导的细胞结合动力学.
  • 证明结合曲线在基于抗原结合域的不同CAR结构之间有所不同.
  • 证实了细胞结合动力学是一致的,抗原特异的,需要活跃的生物信号传递.

结论:

  • 开发的试验提供了一种简单,快速的方法,通过流细胞计量来评估CAR介导的细胞狂热.
  • 这种测定可以应用于各种CAR标,包括与固体瘤和血液癌症相关的标.
  • 该协议能够揭示独特的结合曲线,有助于理解CAR-目标相互作用和近位信号,从而有可能改进基于CAR的疗法.