骨关节炎中的外体细胞:对它们的隔离技术和治疗潜力的审查
Nazmul Huda Syed1,2, Iffath Misbah3, Maryam Azlan2
1Center for Global Health Research, Saveetha Medical Collage and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
Indian journal of orthopaedics
|July 1, 2024
概括
突体,突液中的微小囊泡,通过促进炎症在骨关节炎 (OA) 中发挥作用. 需要新的方法来隔离外体,以获得潜在的OA治疗应用.
科学领域:
- 细胞外囊泡研究研究
- 生物医学科学 生物医学科学
- 再生医学是一种再生医学.
背景情况:
- 外体是细胞分泌的小细胞外囊泡 (30-150 nm),存在于突液中.
- 由于污染物和异质性,它们与复杂的生物流体的隔离具有挑战性.
- 外体细胞通过携带引发巨细胞炎症反应的组件,促进关节骨炎 (OA) 病理生理学.
研究的目的:
- 审查外体生物,隔离技术,以及它们在OA中的作用.
- 探索外体的潜力作为治疗骨关节炎的治疗剂.
主要方法:
- 通过PubMed,Scopus和Web of Science进行了文献搜索.
- 关键词包括"外体"和"骨关节炎".
- 选择了过去15年的文章,包括人类和动物模型.
主要成果:
- 传统的外体隔离方法是艰苦的,耗时的,复杂的.
- 外体与OA过程有关,例如软骨化,关节退化和炎症.
结论:
- 标准化和高通量外体隔离仍然是一个挑战.
- 整合方法可以克服孤立问题.
- 外体显示出对OA的治疗前景,因为它有可能修复软骨,减少炎症和平衡矩阵重塑.
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