层次的全球和局部辅酶信号协调了Arabidopsis中的细胞间数字化
Patricio Pérez-Henríquez1,2, Hongjiang Li1,3, Xiang Zhou4,5
1Institute of Integrated Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
bioRxiv : the preprint server for biology
|July 1, 2024
概括
素通过本地和全球信号通路协调植物组织的发育. 这项研究揭示了核辅酶信号 (TIR1/AFB) 和细胞表面信号 (TMK) 如何相互作用来控制路面细胞形状.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 分子信号传输的方法
背景情况:
- 多细胞组织的发育依赖于协调的细胞极化.
- 阿拉比多普西斯树叶路面细胞 (PCs) 呈现出拼图形状,由auxin信号局部控制.
- 当地和全球协调机制之间的相互作用仍然不清楚.
研究的目的:
- 阐明全球辅酶信号的机制,以协调PC之间的数字化.
- 研究TIR1/AFB途径在全球援助协调中的作用.
- 了解全球和本地辅助信号的层次整合.
主要方法:
- 利用了阿拉比多普西斯突变的遗传分析 (tir1/afb和tmk突变).
- 在本地使用auxin的脱,以评估发育缺陷的救援.
- 在核和细胞表面层面研究了auxin信号通路.
主要成果:
- 在全球范围内,Auxin通过TIR1/AFB核信号通道协调PC间位.
- 一个短暂的auxin最大在cotyledon尖端移动横跨叶子,激活本地PC极化.
- 局部未被关闭的auxin在tir1/afb突变体中挽救了缺陷,但不是tmk突变体,突出了途径的特异性.
结论:
- 全球 (TIR1/AFB-dependent) 和地方 (TMK-mediated) 等级集成的辅助信号控制PC互编字模式.
- 这揭示了协调局部细胞过程与Arabidopsiscotyledons.全组织发育的机制.
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