Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The DNA Replication Fork01:02

The DNA Replication Fork

35.8K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.8K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.7K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.7K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mucin-derived sugars act as metabolic brakes controlling growth initiation in <i>Akkermansia muciniphila</i>.

Gut microbes·2026
Same author

Single-molecule tracking of RNA-DNA hybrid removal enzymes important for lagging-strand replication.

Biophysical journal·2026
Same author

DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.

Critical reviews in biochemistry and molecular biology·2026
Same author

Mapping the rRNA methylome reveals contributions of methyltransferases to ribosome function and antibiotic sensitivity.

bioRxiv : the preprint server for biology·2026
Same author

DnaE uses strand displacement synthesis during Okazaki fragment repair.

bioRxiv : the preprint server for biology·2026
Same author

TlyA is a 23S and 16S 2'-O-methylcytidine methyltransferase important for ribosome assembly in Bacillus subtilis.

Nucleic acids research·2026

相关实验视频

Updated: Jun 22, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K

基因复制启动时间对于保持基因组完整性很重要.

Tristan T Reed1, Abigail H Kendal1, Katherine J Wozniak1,2

  • 1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109.

bioRxiv : the preprint server for biology
|July 1, 2024
PubMed
概括

在Bacillus subtilis中,DNA复制的启动受到严格控制. 低启动和过度启动的DNA复制导致对基因毒性压力的敏感性增加,影响基因组稳定性.

关键词:
这种细菌是 Bacillus subtilis.CcrZ CcrZ 在线阅读复制DNA复制DNA复制DNA复制DnaAA DnaA 是一个这里是ReCAA.

更多相关视频

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K

相关实验视频

Last Updated: Jun 22, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 启动DNA复制是一个由各种因素调节的关键过程.
  • 在 Bacillus subtilis 中,YabA 作为负调节剂,而 CcrZ 激酶则积极调节复制启动.
  • 改变DNA复制启动对基因组稳定性的影响尚未完全理解.

研究的目的:

  • 为了研究DNA复制的后果低启动和过度启动对细菌细菌的基因组稳定性.
  • 为了确定复制启动频率与对基因毒性压力的敏感性之间的关系.

主要方法:

  • 测量源到终点比率作为复制启动活动的代理.
  • 使用ReCA-GFP焦点形成来评估复制叉应力.
  • 在变异复制启动的菌株中评估对基因毒性应激 (mitomycin C) 的敏感性.

主要成果:

  • ΔccrZ突变和某些ccrZ等位基因表现出DNA复制的启动不足.
  • yabA 缺失或 CcrZ 过度生成导致过度启动 DNA 复制.
  • 具有改变复制启动频率的细胞,无论是低启动还是过度启动,都显示出对基因毒性压力的敏感性增加.

结论:

  • 低启动DNA复制可能导致对DNA损伤的敏感性,原因是DNA不足以进行同源重组修复.
  • 过度启动DNA复制会导致复制叉压力,这种压力会因DNA损伤而恶化.
  • 严格控制DNA复制启动频率对于保持基因组稳定性和有效的DNA修复至关重要.