相关实验视频

Updated: Jun 22, 2025

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

3.1K

由MPS1对AURORA A的基于近距离的激活可以加强错误纠正

Nelson Leça, Francisca Barbosa, Sergi Rodriguez-Calado

    bioRxiv : the preprint server for biology
    |July 1, 2024
    PubMed
    概括

    线粒体错误纠正涉及MPS1激活中心体中的Aurora A激酶 (AAK). 这种交叉声确保了正确的染色体对齐,因为它破坏了错误的附着的稳定性,这对于忠实细胞分裂至关重要.

    更多相关视频

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

    10.2K
    Single Molecule Analysis of Laser Localized Psoralen Adducts11:46

    Single Molecule Analysis of Laser Localized Psoralen Adducts

    Published on: April 20, 2017

    6.6K

    相关概念视频

    The Spindle Assembly Checkpoint02:19

    The Spindle Assembly Checkpoint

    The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
    Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
    3.1K
    Anaphase Promoting Complex00:50

    Anaphase Promoting Complex

    The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
    2.8K
    Mismatch Repair01:20

    Mismatch Repair

    Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
    The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
    The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
    4.8K
    DNA Damage can Stall the Cell Cycle02:37

    DNA Damage can Stall the Cell Cycle

    In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
    9.1K
    Long-patch Base Excision Repair01:02

    Long-patch Base Excision Repair

    Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
    7.0K
    Improving Translational Accuracy02:07

    Improving Translational Accuracy

    Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
    10.0K