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在小鼠中使用过多的败血症诱导肌肉病的临床前模型
Franccesco P Boeno1, Diana C Muller1, Ali Aldakkan1
1Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida.
Journal of visualized experiments : JoVE
|July 1, 2024
概括
败血症幸存者经常因为不使用而面临肌肉衰弱. 这项研究介绍了一种新的小鼠模型,模仿卧床患者,以研究败血症引起的肌肉病并测试治疗方法.
科学领域:
- 生物医学研究的研究.
- 败血症的病理生理学
- 骨肌肉生物学 骨肌肉生物学
背景情况:
- 败血症是导致医院死亡的主要原因,幸存者的数量越来越多.
- 幸存者经常出现肌肉衰弱和缩,影响再入院和死亡率.
- 现有的败血症模型缺乏骨肌肉的不使用,这是败血症诱导肌肉病的关键因素.
研究的目的:
- 为新的败血症诱导肌病的小鼠模型提供详细的协议.
- 在临床前环境中复制卧床败血症患者的临床场景.
- 建立一个平台,用于评估治疗策略,防止败血症引起的肌肉缩.
主要方法:
- 在雄性C57Bl/6小鼠中通过结和穿孔 (CLP) 诱导败血症.
- 应用后肢悬浮 (HLS) 七天,从CLP后四天开始,以模拟不使用.
- 肌肉力学和形态与假操作和正常行走控制的比较.
主要成果:
- 开发的模型在小鼠中诱导了显著的肌肉缩和肌肉衰弱.
- 观察到的表型与人类败血症幸存者的表型非常相似.
- 该模型有效地模拟了卧床患者所经历的骨肌肉不使用情况.
结论:
- 本协议建立了有关败血症诱导肌肉病的临床前模型.
- 该模型结合了骨肌肉的不使用,解决了当前研究中的差距.
- 它是测试旨在减轻败血症幸存者的肌肉并发症的干预措施的宝贵工具.
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