最少抗性的途径:由真菌和虫虫植物病原体的非传统效应体分泌
Nawaraj Dulal1, Richard A Wilson1
1Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, U.S.A.
Molecular plant-microbe interactions : MPMI
|July 1, 2024
概括
病原体效应因子分泌依赖于非传统的蛋白质分泌 (UPS) 途径,这些途径受到效应因子基因中的密码使用偏差的影响. 这一发现有助于理解宿主-病原体相互作用和开发抗病策略.
科学领域:
- 植物病理学 植物病理学
- 分子微生物学 分子微生物学
- 简介:真核生物分泌途径
背景情况:
- 主体-病原体的分子相互作用是由效应体分泌的介导,但真核生物的机制仍然不清楚,阻碍了疾病抵抗力.
- 菌和菌菌病原体使用不同的分泌路径:传统的ER-Golgi用于细胞质效应器和非传统的蛋白质分泌 (UPS) 用于细胞质效应器.
- 在Magnaporthe oryzae中UPS通路涉及外囊复合体和t-SNARE用于囊泡融合到等离子膜.
研究的目的:
- 阐明在真核植物病原体中非传统蛋白质分泌 (UPS) 的机制细节.
- 调查子使用在调节细胞质效应体转化和分泌率中的作用.
- 探索这些发现对效应因子发现和增强宿主植物抗病能力的影响.
主要方法:
- 对*Magnaporthe oryzae*中的效应体分泌通路的分析,重点关注Golgi绕过UPS通路.
- 调查mRNA翻译要求,特别是通过tRNA修改解码AA结尾码子.
- 在不同王国的细胞质效应器mRNA中对子使用偏差的比较分析,包括*Phytophthora infestans*.
主要成果:
- 在M. oryzae中,细胞质效应 mRNA 的翻译需要有效地解码由tRNA修改调节的AA结尾编码子.
- 这种转化控制微调效应体分泌率,保持生物的界面复合体完整性和致病性.
- 在整个王国的细胞质效应器mRNA中观察到AA终结的编码子使用偏差,包括在*P. infestans*RxLR-dEER动机序列中,表明保存的翻译速度控制.
结论:
- 植物-真菌界面的完整性和病原性因效应使用而受到令人惊的限制.
- 破解M. oryzae*中UPS的编码码,揭示了一种影响效应体分泌的保存机制.
- 了解非传统的效应体分泌及其与子使用的联系,为效应体发现和疾病抵抗策略提供了新的途径.
关键词:
马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.植物虫 (Phytophthora) 感染了这些植物.激烈的因素 激烈的因素这些是真菌效应器.菌体效应器 菌体效应器这是一种分泌的分泌.更多相关视频
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