氧化与非氧化在已故供体肝脏采购期间冲洗:人类的第一个概念验证研究
Eduardo de Souza Martins Fernandes1,2,3,4,5, Raphael Rodrigues Corrêa6,7, Rodrigo Lopes Leite Furtado6
1Laboratory for Clinical and Experimental Research on Vascular Biology (Biovasc), Department of Physiological Sciences, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Artificial organs
|July 1, 2024
概括
一种新型的低成本设备OxyFlush®在肝移植的静态冷藏 (SCS) 期间维持了腺三酸盐 (ATP) 水平,改善了胆道的保存,但并没有改善肝脏外围细胞的结果.
科学领域:
- 移植免疫学 移植免疫学
- 器官保存技术 器官保存技术
- 生物化学 生物化学
背景情况:
- 肝移植是对末期肝病,肝炎和肝癌的关键治疗方法,面临着全球器官短缺.
- 扩展的捐赠者标准移植越来越多地被使用,需要改进器官保存方法.
- 目前的氧化机器 perfusion 是昂贵的和应用后冷缺血;这项研究探讨了静态冷藏 (SCS) 期间的前 perfusion 氧化.
研究的目的:
- 评估一种新型,低成本的设备 (OxyFlush®),用于在肝脏采购过程中氧化保存溶液.
- 评估设备在SCS期间维持腺三酸盐 (ATP) 水平的能力.
- 为了确定氧化保存溶液对肝脏和普通胆道 (CBD) 完整性的影响.
主要方法:
- 一项随机对照试验,涉及20名患者,分为OxyFlush®和对照组.
- 在器官采购过程中,有氧化保存溶液 (OxyFlush® 组) 与无氧化溶液 (对照组) 相比.
- 在三个时间点收集的肝脏和CBD活检;分析ATP水平和组织学分数;术后实验室测试的评估.
主要成果:
- 在SCS期间,OxyFlush®设备成功地维持了ATP水平.
- 在使用OxyFlush®时,观察到CBD因缺氧而造成的损伤显著改善.
- 没有OxyFlush®对肝膜异位瘤组织学发现或术后实验室测试结果的显著影响.
结论:
- OxyFlush®是一种可行的,低成本的装置,用于在SCS期间保存器官.
- 该技术在减轻缺血性损伤方面显示出前途,特别是在普通胆道中.
- 进一步的研究可能会探索优化其应用以提高整体移植品质.
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