Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Phosphatidylinositol diphosphate binding by ESCRT-III filaments.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets.

bioRxiv : the preprint server for biology·2026
Same author

Improved sensors for fructose-1,6-bisphosphate enable in vivo imaging of glycolysis.

bioRxiv : the preprint server for biology·2026
Same author

IRS4 is a PI3K-activating cancer dependency up-regulated through DNA rearrangements or epigenetic mechanisms in multiple solid tumors.

Science advances·2026
Same author

Parental Knowledge and Decisional Satisfaction Across Genomic Sequencing Consent Processes in Pediatric Cancer.

JCO oncology practice·2026
Same author

Mitochondrial metabolism and signaling direct dendritic cell function in antitumor immunity.

Science (New York, N.Y.)·2026
Same journal

A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

The Journal of cell biology·2026
Same journal

Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

The Journal of cell biology·2026
Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026
Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 22, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

9.1K

一个化补充工具包用于询问脂质滴-有机体相互作用.

Xiao Li1, Rico Gamuyao1, Ming-Lun Wu1

  • 1Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.

The Journal of cell biology
|July 1, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了FABCON,这是一个可视化蜂接触点的新工具. FABCON揭示了脂质滴和有机体之间的动态相互作用,这对能量恒温至关重要.

更多相关视频

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

429
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

10.9K

相关实验视频

Last Updated: Jun 22, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

9.1K
Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

429
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

10.9K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 代谢过程中的代谢.

背景情况:

  • 器官接触点对于细胞脂质和能量平衡至关重要.
  • 难以想象这些纳米尺度的相互作用在活细胞的时间是困难的.

研究的目的:

  • 开发一种用于检测活细胞中有机细胞接触点的新方法.
  • 在不同的代谢条件下研究脂质滴与有机体接触的动态性质.

主要方法:

  • 开发了FABCON (使用splitFAST光蛋白在接触点上激活素的双分子补充).
  • 应用FABCON可视化内分泌网膜 (ER) -线粒体-脂质滴滴接触点.
  • 利用自动化分析管道对接触点进行分类.

主要成果:

  • 通过FABCON,可以对有机体接触部位进行最小扰动的标记.
  • 在脂质滴生物发生和消费过程中,在ER-线粒体-脂质滴接触中显示出动态变化.
  • 按大小分类接触部位,表明不同的调节和功能.

结论:

  • FABCON是一种多功能工具,可用于可视化各种器官接触部位,包括ER-线粒体.
  • 提供了对脂质滴-有机体相互作用的动态调节的新见解.
  • 促进了对代谢调节的进一步机制研究的假设生成.