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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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相关实验视频

Updated: Jun 22, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
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Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

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三维彩虹折射计三维彩虹折射计

Zhiwen Deng, Yingchun Wu, Xinhao Wang

    Optics letters
    |July 1, 2024
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    概括
    此摘要是机器生成的。

    我们介绍了三维彩虹折射计 (TDRR),这是一种用于精确测量滴滴属性的新技术. 该方法使用彩虹光散射精确确定滴滴大小,折射率和3D位置.

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    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
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    相关实验视频

    Last Updated: Jun 22, 2025

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    11:57

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    Published on: May 20, 2013

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    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
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    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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    科学领域:

    • 光学物理学的光学物理.
    • 流体动力学 流体动力学
    • 计量学 计量学 计量学

    背景情况:

    • 彩虹折射计是用于非侵入性滴滴分析的宝贵技术.
    • 现有的方法往往缺乏同时进行3D定位的能力.

    研究的目的:

    • 开发和验证一种新的三维彩虹折射计 (TDRR) 技术.
    • 为了能够准确地测量滴子大小,折射率和轴位置.

    主要方法:

    • 将圆柱形镜头集成到信号采集系统中.
    • 基于射线转移矩阵的TDRR模型的开发.
    • 对彩虹散射角度与系统参数的校准.
    • 实施一个新的彩虹数据处理程序.

    主要成果:

    • 证明彩虹信号倾斜角度与滴滴轴位置相关,使3D定位成为可能.
    • 在用脱离离子水滴进行实验验证方面取得了高准确性.
    • 获得的折射率与<0.0015绝对误差.
    • 测量滴滴大小的误差为<±5%.测量滴滴大小的误差为<±5%.
    • 确定轴位置的误差为<±3%.

    结论:

    • 三维彩虹折射计 (TDRR) 是一种非常准确和有效的滴滴特征化方法.
    • 通过同时提供3D位置信息,TDRR推进了非侵入性滴滴分析.
    • 开发的TDRR技术显示出各种科学和工业应用的巨大潜力.