阅读器 SlYTH2 通过阻碍翻译过程来负面调节番茄果香味
Hanxiao Bian1, Peizhe Song2, Ying Gao1,3
1Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
概括
番茄m6A阅读器 SlYTH2通过控制挥发性合成基因的翻译来调节水果香味. 通过SLYTH2操纵增强香味可以提高消费者对水果的偏好.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 果实质量研究 果实质量研究
背景情况:
- N6-甲基氨酸 (m6A) 是一种关键的RNA修饰,通过m6A阅读器调节基因表达.
- m6A阅读器在水果成熟和质量的作用基本上是未知的.
研究的目的:
- 为了研究番茄m6A阅读器SLYTH2在水果成熟和香味形成中的功能.
- 阐明SLYTH2影响水果质量的分子机制.
主要方法:
- 对SLYTH2.2.的全转录组的m6A结合部位进行分析.
- 分析SLYTH2介导的液体-液体相分离和RNA-蛋白质凝聚物形成.
- 多体形和蛋白质组分析,以评估翻译效率.
- 通过CRISPR编辑生成SLYTH2淘汰番茄.
主要成果:
- SlYTH2与参与挥发性合成的m6A修饰的mRNA (SlHPL,SlCCD1B) 结合,使它们在凝结物中丰富.
- SlYTH2的损失加速了SlHPL和SlCCD1B的翻译,增加了芳香挥发性的产量.
- 用CRISPR编辑的番茄具有增强的香味,显示出消费者偏好增加.
结论:
- 在番茄果实成熟过程中,SLYTH2作为RNA代谢的关键调节者.
- 通过SLYTH2进行m6A介导的调节会影响水果香味和消费者的吸引力.
- 针对m6A路径提供了一种提高水果质量的策略.
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