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通过PDMS打印的纸张喷雾质谱仪检测血液样本中的治疗药物

Lian Xiong¹, Shuang Sun¹, Xinqiong Lu²

¹Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.

|July 1, 2024

概括

一种新型的聚二甲基 (PDMS) 打印纸喷雾装置增强了血液中的治疗药物分析. 这种方法提高了信号强度,并减少了样本损失,以实现更高效的质谱检测.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
生物技术是生物技术.

背景情况:

对复杂的生物样本 (如治疗药物的血液) 的直接分析通常面临着矩阵效应和样本损失的挑战.
传统的纸张喷雾方法可能会受到低效的流体传输的影响,导致信号强度降低.
现有的技术,如电子喷射电离 (ESI),可能不适合直接分析复杂的生物矩阵.

研究的目的:

开发和评估一个聚二甲基西洛 (PDMS) 打印纸喷雾 (PP-PS) 装置,用于血液中治疗药物的增强质谱分析.
在信号强度,检测极限和精度方面,比较PP-PS与传统纸张喷雾和电子喷雾电离 (ESI) 的性能.
评估PP-PS在血液样本中分析精神药物的适用性,使用小型质谱仪.

主要方法:

纸张微流体通道的制造方法是通过直接印聚甲基 (PDMS) 来制造.
在使用PP-PS方法开发的血液样本中分析了五种精神药物 (奥兰扎宾,奎蒂亚平,9-基利,克洛扎宾,利).
将PP-PS与传统纸张喷雾和ESI进行信号强度,相对标准偏差 (RSD),线性和检测极限 (LOD) 的比较.
将PP-PS与自制小型化质谱仪集成在一起,用于现实世界样本分析.

主要成果:

与传统的纸张喷雾相比,PP-PS显著提高了信号强度 (2-5倍),并减少了血液中的精神药物的RSD (2-5.6倍).
与直接血液样本分析的ESI相比,PP-PS显示出更高的信号强度 (9-33倍).
定量分析显示PP-PS的线性范围为5-500 ng/ml,LODs比传统纸张喷雾提高了5-71倍.
通过微型质谱仪使用PP-PS成功检测了所有五种精神药物的前体离子.

结论:

用PDMS打印的喷纸是一种高效的方法,用于在血液等复杂的生物样本中直接分析治疗药物.
在敏感度,效率和降低矩阵效应方面,PP-PS在传统的喷纸和ESI上提供了显著的优势.
开发的PP-PS方法与小型化质谱仪相结合,显示出在传统实验室环境之外的便携式,现场治疗药物监测的巨大潜力.

关键词:

质谱测量质量谱测量纸张微流体在纸张上的作用.纸张喷雾剂是一种纸张喷雾剂.治疗药物监测治疗药物监测

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