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Updated: Jun 22, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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在大肠杆菌中使用Lambda-Red重组组合的无痕百科病毒基因组编辑.

Linda A de Jong1, Linda van Oosten1, Gorben P Pijlman2

  • 1Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.

Methods in molecular biology (Clifton, N.J.)
|July 1, 2024
PubMed
概括

这项研究引入了一种无痕的基因组编辑方法,用于大肠杆菌中的baculovirus DNA,从而实现精确的基因修改,以改善生物农药和重组蛋白质生产.

关键词:
巴克米德是一种细菌.百科病毒表达载体系统删除 删除 删除基因组编辑 基因组编辑突变发生是突变发生的.变异点的变异点是指变异点的变化点.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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