导航黑毛羊 (Ovis aries) 的染色体级基因组组合
Zengkui Lu1,2, Chao Yuan1,2, Xuejiao An1,2
1Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, 730050, China.
Scientific data
|July 1, 2024
概括
研究人员对指南黑毛羊 (GD) 的基因组进行了测序,这是一种适应高海拔地区的藏族羊品种. 这种高质量的参考基因组为它们独特的适应提供了关键的见解,并有助于未来的遗传改进.
科学领域:
- 基因组学就是基因组学.
- 动物遗传学动物遗传学
- 高海拔适应 高海拔适应
背景情况:
- 黑毛羊 (GD) 是藏族羊 (Ovis aries),居住在高海拔地区 (>4,000米).
- 有限的基因组数据阻碍了对它们高海拔适应机制的理解.
研究的目的:
- 测序和组装一个高质量的参考基因组为指南黑毛羊.
- 为研究高海拔适应和基因改进提供基础资源.
主要方法:
- 利用PacBio,Hi-C和Illumina测序技术进行基因组组装.
- 基因组组装大小: 2.73 Gb; 孔蒂格N50: 20.30 Mb; 脚手架N50: 107.63 Mb.
- 使用BUSCO评分 (93.1%) 评估基因组完整性.
主要成果:
- 组装了一个2.73Gb的基因组,具有很高的连续性和支架N50.
- 确定了20759个编码蛋白质的基因,具有98.42%的功能注释.
- 重复的元素约占基因组的52.2%.
结论:
- 高质量的GD基因组组装是一个显著的进步.
- 这一资源将促进对西藏绵羊高海拔适应性的研究.
- 能够为这种品种提供未来的分子育种和遗传改进策略.
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