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采样时间对于微生物组分析的可复制性至关重要

Celeste Allaband^1,2,3, Amulya Lingaraju², Stephany Flores Ramos^1,2,3

¹Division of Biomedical Sciences, University of California, San Diego, La Jolla, CA, USA.

Nature metabolism

|July 1, 2024

概括

微生物组研究受到样本采集时间的影响,这导致了变化,并影响了比干预更大的结果. 稳定的时间对于可靠的微生物组研究至关重要.

科学领域:

微生物学微生物学
基因组学就是基因组学.
动物研究动物研究

背景情况:

微生物组研究正在向机理学和干预研究迈进.
尽管有改善,但仍存在无法解释的变异性和缺乏可复制性.
微生物群中的日间振荡是一个未被充分研究的因素.

研究的目的:

调查样本采集时间对微生物组研究结论的影响.
为了比较样本采集时间的影响大小与实验干预和饮食变化.
突出日间节律在微生物群变异性和可复制性中的作用.

主要方法:

对16S安普利康测序研究的回顾性分析.
专注于涉及雄性小鼠的研究.
基于采样时间点对数据的检查.

主要成果:

样本采集时间显著影响微生物组研究结论.
采集时间的效果大小大于每日干预或饮食改变.
即使收集时间有4小时的差异,也可能导致不同的结论.
群体之间的微生物组分歧的时间是实验特异的.

结论:

不一致的样本采集时间是微生物组研究中交叉研究可复制性差的可能原因.
标准化样本采集时间对于准确的微生物组分析至关重要.
昼间节律对其他研究领域的影响值得研究.

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