阿拉比多的PROTODERMAL FACTOR2结合了酸胆,并通过转录来调节脂代谢
Izabela Wojciechowska1, Thiya Mukherjee2,3,4, Patrick Knox-Brown5
1Max Planck Institute of Molecular Plant Physiology, 14476, Potsdam, Germany.
The New phytologist
|July 2, 2024
概括
植物转录因子PROTODERMAL FACTOR2 (PDF2) 通过它们的START域来招募溶解酸丁胆. 这种脂质结合调节基因表达和蛋白质稳定性,将脂质状态与酸盐可用性结合起来.
科学领域:
- 植物分子生物学 植物分子生物学
- 脂质新陈代谢 脂质新陈代谢
- 基因调节 基因调节
背景情况:
- 植物家庭主体氨酸拉链IV (HD-Zip IV) 转录因子 (TFs) 具有与类固醇急性调节蛋白 (StAR) 相关的脂质转移 (START) 域.
- START域的确切功能,特别是它作为TF活动中的脂质传感器的作用,仍然不清楚.
研究的目的:
- 调查START域在HD-Zip IV TF功能中的作用.
- 为了识别由PDF2转录因子招募的配体.
- 阐明脂质结合影响TF活性并与营养素可用性结合的机制.
主要方法:
- 从Arabidopsis细胞培养物中进行并列亲和性净化,以识别相互作用的蛋白质.
- 微尺度热泳试验用于评估连接体结合亲和力.
- 与脂和酸盐饥饿有关的基因表达的分析.
- 循环胺追逐实验,以评估蛋白质的稳定性.
主要成果:
- 蛋白体因子2 (PDF2) 通过依赖START域的方式招募溶解酸胆 (LysoPCs).
- 预测的结合位的突变减少了LysoPC的结合.
- PDF2调节参与脂和酸盐 (Pi) 饥饿的基因,并与一个Pi-响应元素结合.
- pdf2突变体表现出改变的脂平衡和在Pi饥饿下增长.
- START域结合影响蛋白质的稳定性,Pi饥饿增强了蛋白质的不稳定性.
结论:
- 在PDF2的START域的功能作为一个传感器的膜脂状态在表皮.
- 通过PDF2的脂质结合是一种控制TF活性并将脂质代谢与酸盐可用性和基因表达结合的机制.
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