通过级联生物转换进行绿色和酶选择性合成:从简单的 Racemic 基质到高价值的 chiral 化学物质
Jieran Yi1, Nicholas Jun Jie Goh1, Zhi Li1
1Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.
Chemistry, an Asian journal
|July 2, 2024
概括
酶级联生物转化提供了一种更绿色,高度酶选择性的方法,用于从racemic基质合成奇拉化学物质. 本综述探讨了有效的性化学生产的策略,应用和未来的酶工程进步.
科学领域:
- 生物催化剂和绿色化学
- 酶合成酶的合成
- 奇拉尔化学生产 奇拉尔化学生产
背景情况:
- 传统的不对称合成往往涉及多步反应,恶劣的条件和昂贵的性联结体,导致低的酶选择性和浪费.
- 酶催化为生产性化合物提供了一种可持续和高度酶选择性的替代方案.
- 级联生物转化使单多步反应成为可能,最大限度地减少中间隔离和废物产生.
研究的目的:
- 审查构建酶级联生物转换的一般策略.
- 为突出应用在转化racemic基质到有价值的性化学品.
- 批判性地评估当前的酶级联系统,并建议未来的改进.
主要方法:
- 对三种一般策略的回顾: enantio-convergent反应,动态动力学分辨率,以及破坏和重新安装奇拉性.
- 分析将racemic环氧化物,酒精和酸转化为奇拉性氨基酒精,酸和氨基的例子.
- 批判性审查产品度,反体过量 (ee),产量,可扩展性和基质范围.
主要成果:
- 酶级联有效地将简单的racemic基质转化为有价值的性化学物质,具有高产量和高效率.
- 通过各种策略,可以合成多种奇拉产物,如氨基醇,氧酸和氨基.
- 目前的布在产量,效率和可扩展性方面表现有前途,并有优化空间.
结论:
- 酶级联生物转化是可持续性性化学合成的强大工具.
- 酶工程的进一步改进,包括人工智能引导的定向进化和微流体学,可以提高催化效率和实际适用性.
- 这些进展将加速开发更绿色,更具成本效益的途径,以获得制药行业必不可少的性化合物.
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