纳米粒子辅助的管组装是环境依赖的
Mahima Unnikrishnan1, Yuhan Wang2, Martin Gruebele1,2,3,4
1Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801.
概括
纳米颗粒在实验室的缓冲区中促进细菌管二分化,但在哺乳动物细胞内影响最小. 这突出了在没有表面功能化的情况下将体外纳米材料效应转化为复杂的细胞环境的挑战.
科学领域:
- 生物物理学的生物物理.
- 纳米医学是一种纳米医学.
- 细胞生物学 细胞生物学
背景情况:
- 纳米材料与生物系统相互作用,形成生物分子冠状,改变蛋白质的行为.
- 研究纳米粒子对蛋白质相互作用的影响通常发生在缓冲器中的ex vivo,缺乏体内细胞背景.
- 来自Prosthecobacter的细菌管蛋白 (BtubA/BtubB) 与真核细胞管相似地自组装.
研究的目的:
- 在实验室中量化比较二氧化纳米颗粒对细菌蛋白 (BtubAB) 蛋白协会的影响,与活哺乳动物细胞中的效果.
- 研究蛋白冠在调解纳米粒子-蛋白相互作用中的作用.
- 评估体外发现对细胞环境的可翻译性.
主要方法:
- 在BtubA和BtubB单体上使用了mEGFP和mRuby3光标签的Förster共振能量转移 (FRET).
- 使用光光谱和显微镜测量了BtubAB的二分化和自我组装.
- 在无细胞缓冲区和哺乳动物细胞中,向BtubAB引入了非功能化的二氧化纳米粒子.
主要成果:
- 纳米粒子显著增强了缓冲区中的BtubAB二分化,这归因于蛋白质冠状形成.
- 相比之下,这些纳米颗粒对哺乳动物细胞环境中的细菌氨酸自我组装具有极小的影响.
- 复杂的细胞环境,与竞争的生物分子,影响纳米粒子-蛋白质相互作用.
结论:
- 在体外观察到的纳米粒子诱导的蛋白质与蛋白质相互作用可能不会直接转化为细胞环境.
- 缓冲区中的蛋白冠状形成增强了相互作用,但由于竞争性结合,这种效应在体内减少了.
- 纳米颗粒的表面功能化对于有针对性的相互作用和克服复杂生物系统中的竞争性结合至关重要.
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