在超长螺旋通道中稳定和加速二次流,用于高通量细胞操纵
Shaofei Shen1, Xufang Liu1, Kuohai Fan2
1Shanxi Key Lab for Modernization of TCVM, College of Life Science, Shanxi Agricultural University, Taiyuan, Shanxi 030000, P. R. China.
Analytical chemistry
|July 2, 2024
概括
这项研究引入了一种新的螺旋微流体装置,用于高效的细胞操纵. 该设备稳定了二次流量,使血液和癌细胞的高吞吐量处理能够用于生物分析.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 细胞生物学 细胞生物学
背景情况:
- 有效的细胞操纵对于生物分析和医学诊断至关重要.
- 目前的方法面临的局限性是由于不稳定的二次流量和低吞吐量.
- 惯性微流体设备具有潜力,但需要优化以实现实际应用.
研究的目的:
- 开发一种创新的惯性微流体装置,用于高通量细胞操纵.
- 用几何限制和微观结构在微通道内稳定和加速二次流动.
- 为了证明在广泛的吞吐量范围内有效操纵血液和癌细胞.
主要方法:
- 设计和制造具有有序微观结构的螺旋惯性微流体装置.
- 利用几何限制来调节类似迪恩的二次流动.
- 测试设备的性能与血液细胞和癌细胞在各种输出.
主要成果:
- 设计的螺旋通道成功地稳定并加速了二次流动.
- 实现了血液细胞 (1.73 × 10^8到1.16 × 10^9细胞/分钟) 的高通量操纵.
- 证明有效地操纵癌细胞 (0.5 × 10^6到5 × 10^7细胞/分钟).
结论:
- 开发的微流体装置克服了细胞操纵现有技术的局限性.
- 稳定和加速二次流的方法使高通量细胞处理成为可能.
- 这项技术对生物医学分析具有前景,特别是对于大量的液体样本.
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