纤维细胞激活蛋白-α在人类深静脉血栓症中的表达
Nobuyuki Oguri1, Toshihiro Gi1, Eriko Nakamura1
1Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Thrombosis research
|July 2, 2024
概括
纤维细胞激活蛋白-α (FAP) 在组织深静脉血栓症 (DVT) 组织中发现,主要在纤维细胞和肌纤维细胞内. 在低增殖性纤维细胞中,FAP的表达更高,这表明在DVT进展中发挥了作用.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 病理学 病理学 病理学
背景情况:
- 纤维细胞激活蛋白-α (FAP) 是一种涉及伤口愈合,纤维化和癌症的血清蛋白酶.
- 它在深静脉血栓形成 (DVT) 中的作用以前尚未确定.
- 这项研究旨在阐明FAP在DVT中的表达和局部化.
研究的目的:
- 在人类深静脉血栓塞 (DVT) 中调查FAP的存在和细胞局部.
- 在与血栓形成相关的不同条件下分析纤维细胞中的FAP表达.
- 了解FAP在DVT的发病过程中的潜在作用.
主要方法:
- 对14个患者衍生的DVT血栓进行了病理分析,将其分类为新鲜,溶解和组织成分.
- 免疫组合化学和双免疫光染色以检测FAP表达和与细胞标记物共定位.
- 在体外培养皮肤纤维细胞以评估FAP mRNA和蛋白质水平在不同的血清度和刺激 (血红素,血) 下.
主要成果:
- 所有的DVT血栓呈现异质的组成,其中83%含有新鲜和有组织的成分.
- FAP表达局部化到瘤组织区域.
- FAP主要由维门阳性和α-平滑肌肉活性蛋白阳性纤维细胞表达,以及一些CD163阳性巨细胞.
- 与10%的FBS相比,低增殖活性纤维细胞 (培养在0.1%的FBS中) 的FAP mRNA和蛋白质水平显著更高.
- 血红素补充剂在10%的FBS培养的纤维细胞中降低了FAP mRNA水平,而血栓素没有显著影响.
结论:
- 静脉血栓的异质性表明DVT中的复杂,多步骤的形成过程.
- 纤维细胞和肌纤维细胞在DVT的组织阶段可能是FAP的来源.
- FAP表达似乎与纤维细胞的增殖活动相反相关,这表明它可能在DVT中的纤维化过程中发挥作用.
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