Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection.

Molecular cell·2026
Same author

High-resolution mapping of CCR4-NOT recruitment elements reveals transcriptome-wide drivers of mRNA decay.

Cell reports·2026
Same author

Comprehensive RNA-binding protein analyses and deep learning uncover genetic constraints and disease associations in protein-RNA interfaces.

Cell systems·2026
Same author

Flipper: An advanced framework for identifying differential RNA binding behavior with eCLIP data.

bioRxiv : the preprint server for biology·2026
Same author

System-level measurement, modeling, and manipulation of RNA.

RNA (New York, N.Y.)·2026
Same author

RNA-binding protein LARP6 coordinates hepatic stellate cell activation and liver fibrosis.

The Journal of clinical investigation·2026

相关实验视频

Updated: Jun 22, 2025

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

58.4K

泥者在多重复合的CLIP数据中检测到组合RNA结合蛋白相互作用.

Hsuanlin Her1, Katherine L Rothamel2, Grady G Nguyen2

  • 1Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Sanford Stem Cell Institute Innovation Center and Stem Cell Program, University of California, San Diego, La Jolla, CA 92093, USA; Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Bioinformatics and Systems Biology Graduate Program, University of California, San Diego, La Jolla, CA 92093, USA.

Cell genomics
|July 2, 2024
PubMed
概括

Mudskipper通过改进多重交联和免疫沉降 (CLIP) 实验的数据分析来增强RNA结合蛋白 (RBP) 站点映射. 这种计算工具可以提高RBP绑定位点识别的精度和回忆.

关键词:
这就是CLIP CLIP.这是一个RNARNARNARNARNA.RNA结合蛋白质是RNA结合的蛋白质.深度学习是一种深度学习.基因调节 基因调节 基因调节拼接 拼接 拼接 拼接翻译学 翻译学 翻译学 翻译学变体解释变体解释

更多相关视频

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

53.4K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.0K

相关实验视频

Last Updated: Jun 22, 2025

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

58.4K
PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

53.4K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.0K

科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 了解蛋白质-RNA相互作用对于破译RNA处理和调节至关重要.
  • 多重交联和免疫沉 (CLIP) 技术,如抗体条形编码的eCLIP (ABC),已经推进了RNA结合蛋白 (RBP) 结合部位的高通量映射.
  • 现有的多重CLIP数据集由于其多变量性质和不同RBPs的可变信号噪声比率而存在挑战.

研究的目的:

  • 开发一个计算套件,Mudskipper,用于改进多重CLIP数据集的分析.
  • 为了应对多变量数据和非统一的信号噪声比在绘制RBP绑定站点的挑战.
  • 提高RBP结合位点识别的精度和回忆,特别是对于低信号噪声比的RBP.

主要方法:

  • 开发Mudskipper,一个计算套件,有两个核心组件.
  • 实施迪里克莱多项混合模型来处理ABC数据集的多变量特征.
  • 应用软掩盖方法来识别和去除非特异性蛋白质-RNA相互作用.

主要成果:

  • 与多重CLIP数据集上的现有工具相比,Mudskipper表现出更高的精度和回忆能力.
  • 该计算套件有效地分析重复元素和小型非编码RNA.
  • 泥者有助于解开拼接结果和变异相关的破坏.

结论:

  • Mudskipper提供了一种多功能和有效的计算解决方案,用于分析复杂的多重Clip数据.
  • 该工具增强了对RNA结合蛋白质介导调节和疾病机制的调查.
  • Mudskipper 能够对蛋白质-RNA 相互作用进行更高吞吐量和更准确的研究.