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相关概念视频

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

576
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Immunofluorescence Microscopy01:12

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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相关实验视频

Updated: Jun 22, 2025

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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基于光纤的光检测. 第一部分:基本概念

Bong Lee1, Luca Ceresa1, Danh Pham1

  • 1Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, 76109, United States of America.

Methods and applications in fluorescence
|July 2, 2024
PubMed
概括
此摘要是机器生成的。

光纤可实现紧的现场检测,但信号扭曲阻碍了准确的分析. 这项研究模拟了收集光效的效率,以纠正光谱数据,在各种应用中提高准确性.

关键词:
吸收 吸收 吸收 吸收光纤光学纤维是一种光纤.光是一种光.内部波器效应的内部波器效应频谱学是一种光谱学.

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相关实验视频

Last Updated: Jun 22, 2025

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科学领域:

  • 分析化学 分析化学
  • 生物医学光学 生物医学光学
  • 光学工程是指光学工程.

背景情况:

  • 光纤对于工业和生物医学环境中的紧,现场化学和生物分析至关重要.
  • 目前对光收集效率和光纤系统中检测灵敏度的理解是有限的.
  • 在比较具有不同光学特性的样品和纠正内部波器效应和散射等信号元件方面存在挑战.

研究的目的:

  • 调查导致光纤检测信号和光谱扭曲的因素.
  • 开发一种对激发特征和排放收集效率的通用模型.
  • 为纠正测量频谱和恢复真实排放配置文件提供一个框架.

主要方法:

  • 在裸体和镜头合的平底光纤光纤配置中检测信号的分析.
  • 开发和实验验证光收集的通用模型.
  • 评估影响信号/光谱扭曲的参数.

主要成果:

  • 确定了导致光纤测量中信号和光谱扭曲的关键因素.
  • 提出了一种经过验证的模型,准确地描述了激发概况和排放收集效率.
  • 证明了该模型在理解和潜在地纠正光谱文物方面的实用性.

结论:

  • 对光纤信号采集的基本理解对于准确的定量分析至关重要.
  • 开发的模型为纠正光谱数据提供了一个合理的基础,特别是对于光密样本.
  • 改进的光谱校正将提高不同实验设置中的结果的可靠性和可比性.