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Updated: Jun 22, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

孕产妇mRNA死亡化在体外成熟的小鼠和人类卵细胞中是有缺陷的

Yusheng Liu^1,2, Wenrong Tao³, Shuang Wu^4,5

¹College of Life Science, Northeast Forestry University, Harbin, 150040, China. liuys1126@foxmail.com.

Nature communications

|July 2, 2024

概括

卵细胞在体外成熟导致母体mRNA死亡化受损,这是辅助生殖的一个关键缺陷. 这是由于死乙酶机制的问题造成的,影响成熟卵细胞中的基因表达.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
辅助生殖技术是一种辅助生殖技术.

背景情况:

卵细胞体外成熟 (IVM) 是辅助生殖技术 (ART) 的基石.
有相当数量的基因在体外成熟 (IVM) 基因相 II (MII) 卵细胞中表现出异常的高表达,与它们在物种中的体内对应物相比.
驱动IVM卵细胞表达变化的分子机制在很大程度上是未知的.

研究的目的:

研究在体外成熟卵细胞中异常基因表达背后的机制.
在体外与体内的卵细胞成熟过程中概述转录组全方位的poly (A) 尾动态.
为了确定有助于改变IVM卵细胞中的母性mRNA丰度的特定分子缺陷.

主要方法:

使用了包括多A的RNA异形序列 (PAIso-seq) 来分析多A的尾巴长度.
在体内和体外成熟的小鼠和人类卵子细胞的转录组比较.
研究了死化机械元件的表达和功能.

主要成果:

在实验室成熟的MII卵细胞表现出母体mRNA的死亡化受损,导致它们的丰度增加.
在IVM卵细胞中,Btg4和Cnot7mRNA的细胞质多化,对于化至关重要,是有缺陷的.
死亡酶成分的翻译减少导致母体mRNA死亡酶的总体缺陷.

结论:

发育不良的母性mRNA死亡化是一种在体外成熟的卵细胞中明显的分子缺陷.
死亡化机制中的这个缺陷导致IVM卵细胞中观察到的基因表达模式的改变.
了解这种机制对于提高辅助生殖技术的有效性和安全性至关重要.

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