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Updated: May 6, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

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在Kluyveromyces marxianus中使用SlugCas9-HF有效和无标记基因集成

Huanyu Zhou1,2, Tian Tian2, Jingtong Liu1,2

  • 1Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Fudan University, Shanghai, 201399, China.

Communications biology
|July 2, 2024
PubMed
概括

我们开发了一个CRISPR-Cas9工具包,用于精确的多基因集成Kluyveromyces marxianus酵母. 这种合成生物学工具通过有效的基因组工程来实现复杂的途径重建,从而加速工业应用.

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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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